孕鼠暴露双酚A对仔鼠存活率、生殖激素及相关基因的影响  

Effects of Maternal Exposure to Bisphenol A on Survival Rate,Reproductive Hormones and Relative Genes of Offspring Mice

作　　者：马双[1] 宋鹏琰 翟福展 钟秀会[1,2] 

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]河北农业大学中兽医学院,保定071001

出　　处：《中国畜牧兽医》2017年第7期2033-2041,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技部十二五科技支撑计划课题"防治家畜胎产病和提高畜禽免疫力中兽药创制及应用示范"(2011BAD34B02)

摘　　要：为了研究孕鼠在孕期暴露双酚A(bisphenol A,BPA)对仔鼠生殖激素及相关基因的影响,试验将40只昆明孕鼠随机分为A、B、C、D共4组,每组10只。其中A组为对照组,饲喂普通鼠粮;B、C、D组孕鼠整个妊娠期(妊娠1d至分娩)分别按每只鼠每天50、500、2 500mg/kg体重给予BPA,待孕鼠自然分娩,观察记录仔鼠死亡情况。至仔鼠性成熟(56日龄)剖杀仔鼠,摘取睾丸或卵巢称重并计算脏器指数,HE染色观察卵巢或睾丸组织结构的变化,ELISA试剂盒分析仔鼠血清睾酮(T)、促卵泡素(FSH)及雌二醇(E2)水平,免疫组织化学方法检测仔鼠睾丸或卵巢Bax、Bcl-2蛋白的表达,实时荧光定量PCR检测仔鼠睾丸StAR、CYP11a或卵巢AMH、Kitlg mRNA表达。结果显示,孕鼠暴露BPA极显著增加了仔鼠死亡率(P<0.01),显著降低了仔鼠睾丸重(P<0.05)。ELISA检测结果表明,孕鼠暴露BPA极显著降低了仔鼠T(♂)及FSH(♀)含量(P<0.01),极显著升高了仔鼠(♀)E2水平(P<0.01)。HE染色结果显示,随BPA剂量增加,仔鼠睾丸组织损伤严重,间质细胞减少;卵巢组织结构随BPA剂量增大,空泡逐渐增多,黄体颗粒数量逐渐减少。免疫组化结果显示,孕鼠暴露BPA增加了仔鼠睾丸或卵巢组织中Bax阳性蛋白表达,减少了Bcl-2阳性蛋白表达,显著降低了雄性仔鼠StAR mRNA表达量(P<0.05);B、D组雄性仔鼠CYP11amRNA表达量极显著降低(P<0.01),而C组CYP11amRNA表达量极显著升高(P<0.01);C、D组雌鼠Kitlg mRNA表达极显著降低(P<0.01),AMH mRNA表达量显著升高(P<0.05)。本试验结果表明,孕鼠妊娠期暴露不同剂量BPA增加了仔鼠死亡率,扰乱了生殖激素平衡和睾丸/卵巢中相关凋亡蛋白及生殖基因表达。This study was aimed to investigate the reproductive toxicity of bisphenol A（BPA）exposed to the mother on the offsprings mice.Forty pregnant Kunming mice were randomly divided into 4groups,i.e.groups A,B,C and D with 10 mice in each group.Group A was the control group and the mice received conventional feeds,mice in groups B,C and D were given 50,500and2 500mg/kg BW BPA in feedstuffs during the whole gestation period（from 1dto parturition）,respectively.The death rates of the offsprings were calculated every week.The offspring mice were sacrificed at 56 days of age（at puberty）.The morphology of ovary and testicular tissueswere observed with hematoxylin-eosin（HE）staining.The levels of estradiol（E2）,follicle stimulating hormone（FSH）,testosterone（T）in mice serum were detected with ELISA Kit.The protein levels of Bax and Bcl-2in ovary or testicular tissues were detected with immunohistochemistry,and the StAR,CYP11 amRNA levels in testicular tissues,the AMH,Kitlg mRNA levels in ovary were measured using Real-time PCR.The results showed that exposure of BPA to the mother extremely significantly increased the mortality（P0.01）,and significantly reduced the testicular weight of offspring mice（P0.05）.Maternal exposure to BPA extremely significantly reduced the levels of T（♂）and FSH（♀）（P0.01）,and extremely significantly elevated E2（♀）level in offspring mice（P0.01）.BPA exposure damaged the testicular with less leydig cells and ovarian tissues with more vacuoles and less corpus granules in offspring mice.Immunohistochemistry results revealed that maternal exposure to BPA increased the Bax protein level and decreased the Bcl-2protein level of testicular and ovary tissues in offspring mice.BPA significantly reduced the StAR mRNA expression in male offsprings（P0.05）.However,the mRNA level of CYP11 ain groups B and D extremely significantly decreased while group C showed an significant elevation in male offsprings（P0.01）.The expression levels of Kitlg mRNA in gr

关 键 词：双酚A 小鼠 生殖激素 免疫组织化学 生殖基因 

分 类 号：Q579.13[生物学—生物化学]