不同剂量胡椒碱对C57BL6小鼠胆固醇结石作用的影响  

Effect of Different Doses of Pepper on Cholesterol Gallstone in C57BL6 Mice

作　　者：张文冲 程嘉艺[1] 宁天一[1] 李勇男[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]中国医科大学附属盛京医院第二普通外科,辽宁沈阳110004

出　　处：《辽宁中医杂志》2017年第7期1496-1498,I0002,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81200318)

摘　　要：目的:观察不同剂量的胡椒碱对C57BL/6小鼠胆固醇结石形成的影响。方法:将C57BL/6小鼠70只,随机分为空白对照组、模型组、胡椒碱20 mg·kg^(-1)剂量组、胡椒碱40 mg·kg^(-1)剂量组、胡椒碱60 mg·kg^(-1)剂量组、胡椒碱90 mg·kg^(-1)剂量组、胡椒碱120 mg·kg^(-1)剂量组7组。应用全自动生化分析仪测血清中的总胆固醇(TC)、甘油三酯(TG)的含量。结果:与空白对照组(2.4740±0.2884)比较,模型组(3.3420±0.2266)小鼠总胆固醇含量明显升高,与模型组(3.3420±0.2266)比较,胡椒碱20 mg·kg^(-1)剂量组(2.6509±0.4458)、胡椒碱40 mg·kg^(-1)剂量组(2.4090±0.5843)小鼠总胆固醇含量明显降低;与模型组(0.9010±0.1694)比较,胡椒碱20 mg·kg^(-1)剂量组(1.3358±0.5908)、胡椒碱40 mg·kg^(-1)剂量组(1.7409±0.5797)小鼠甘油三酯含量明显升高,胡椒碱120 mg·kg^(-1)剂量组(0.6530±0.1578)小鼠甘油三酯含量明显降低。结论:胡椒碱抑制C57BL/6小鼠胆固醇结石的形成与胡椒碱的剂量相关,胡椒碱剂量为40 mg·kg^(-1)时,抑制小鼠胆固醇结石的作用最强。但是胡椒碱剂量为120 mg·kg^(-1)可明显促进小鼠体内甘油三酯的代谢,并具有减轻体重的作用趋势。Objective：To observe the effect of different doses of pepper on cholesterol gallstone formation in C57BL/6 mice.Methods：Seventy C57BL/6 mice were randomly divided into seven groups：blank control group,model group,piperine 20 mg·kg^（-1）dose group,piperine 40 mg·kg-1dose group,piperine 60 mg·kg-1 dose group,piperine 90 mg·kg-1 dose group and piperine 120 mg·kg-1dose group.Total cholesterol（TC） and triglyceride（TG） in serum were measured by automatic biochemical analyzer.Results：Compared with the blank control group＇s（2.4740±0.2884）,model group＇s total cholesterol content（3.3420 ±0.2266） increased significantly.Compared with the model group＇s（3.3420±0.2266）,piperine 20 mg·kg-1 dose group＇s total cholesterol content（2.6509±0.4458）,piperine 40 mg·kg-1 dose group＇s（2.4090±0.5843） decreased significantly and compared with model group＇s（0.9010±0.1694）,piperine 20 mg·kg-1dose group＇s triglyceride content（1.3358±0.5908） and piperine 40 mg·kg-1 dose group＇s（1.7409±0.5797） increased significantly.Piperine 120 mg·kg-1dose group＇s triglyceride content（0.6530±0.1578） in mice decreased significantly.Conclusion：The formation of cholesterol gallstone formation in C57BL/6 mice was related to the dose of piperine and the effect of 40 mg·kg-1 was the best.But the dose of 120 mg·kg-1 can significantly promote the metabolism of triglyceride in mice and has a tendency to reduce body weight.

关 键 词：胡椒碱 胆固醇 胆固醇结石 甘油三酯 

分 类 号：R285.5[医药卫生—中药学]