高效液相色谱法测定利福昔明混悬乳剂的含量  

Content Determination of Rifaximin Suspo-emulsions by HPLC

作　　者：朱庆贺 苗艳[1] 王爽[1] 陈曦[1] 王观悦[1] 史同瑞[1] 

机构地区：[1]黑龙江省兽医科学研究所,齐齐哈尔161006

出　　处：《中国兽药杂志》2017年第7期42-46,共5页Chinese Journal of Veterinary Drug

基　　金：黑龙江省应用技术研究与开发项(czznkt2016-2)

摘　　要：建立了测定利福昔明混悬乳剂含量的高效液相色谱法(HPLC)。采用C18色谱柱(4.6 mm×150 mm,5μm),以甲醇-乙腈-0.075 mol/L磷酸二氢钾溶液-1 mol/L柠檬酸溶液(32∶28∶25∶4)为流动相,流速1.0 m L/min,检测波长236 nm,进样量20μL,柱温25℃。利福昔明浓度在5~100μg/m L时,其峰面积与浓度线性关系良好(r2=0.9996),无杂质干扰,稳定性高,回收率范围为98.4%~100.2%,RSD为0.58%~1.32%。本操作方法简便、分析快速、结果准确,适用于利福昔明混悬乳剂的含量测定。A method for the content determination of rifaximin suspoemulsion by HPLC was established. HPLC system consisted of C18 column （ 5μm,4.6×150 mm） , methyl aleohol-aeetonitrile-citric acid-potassium phosphate monobasic （32 ： 28 ： 25 ： 4） as mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 236 nm. The injection volume was 20 p,L and column temperature was room temperature. There was no interference occurred from inpurities. The calibration curve was linear in the range of 5-100 μg/mL （r2= 0.9996）.The recovery was 98.4%- 100.2% and RSD was 0.58%- 1.32%. The method is rapid, simple and accurate for the content determination of rifaximin suspo-emulsions.

关 键 词：利福昔明 混悬乳剂 高效液相色谱法 含量 

分 类 号：S859.79[农业科学—临床兽医学]