两种AM真菌对冬小麦根系锌吸收的影响  

Effects of two AM fungi on zinc uptake of winter wheat roots

作　　者：马晓娜[1,2] 罗万清[1,2] 许飞飞[1,2] 吴福勇[1,2] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]农业部西北植物营养与农业环境重点实验室,陕西杨凌712100

出　　处：《菌物学报》2017年第7期933-941,共9页Mycosystema

基　　金：国家自然科学基金(41571456);中央高校基本科研业务费专项资金(2452016118)~~

摘　　要：无论在温室还是在大田条件下,菌根(AM)真菌均能够侵染冬小麦,但其对冬小麦锌(Zn)吸收的效应及机制尚不明确。本研究将摩西管柄囊霉Funneliformis mosseae和根内根孢囊霉Rhizophagus intraradices分别接种于冬小麦Triticum aestivum(品种:小偃22)根围,10周后分别利用菌根化幼苗进行短期(0–90min)Zn吸收动力学试验和长期(0–210min)Zn吸收积累试验,并研究AM真菌侵染对冬小麦根系形态特征的影响。结果表明,AM真菌通过增加根系长度和根尖数量来扩大冬小麦根系Zn吸收的有效面积、Zn最大吸收速度V_(max)和Zn^(2+)流入根系的速度α,进而促进冬小麦根系对Zn的吸收。接种摩西管柄囊霉降低了K_m值而接种根内根孢囊霉增加了K_m值,这可能与不同AM真菌对冬小麦根系形态影响及对Zn转运蛋白基因表达的影响存在差异有关。Arbuscular mycorrhizal（AM） fungi could colonize winter wheat either under greenhouse or field conditions. However, the effects of AM fungi on Zn uptake in the winter wheat are still not fully elucidated. In this study, Funneliformis mosseae and Rhizophagus intraradices were inoculated to rhizosphere of the winter wheat Triticum aestivum（cultivar： Xiaoyan 22） respectively. After ten weeks of growth, a short-term（0–90min） uptake kinetics experiment and a long-term（0–210min） Zn accumulation experiment were conducted using seedlings of winter wheat colonized by AM fungi. The effects of AM fungi on root morphology of winter wheat have also been investigated. The results showed that AM fungi could increase the effective area of Zn uptake in the winter wheat roots by increasing root length and root tip numbers, and the maximum absorption rate（Vmax） and the rate of Zn^2＋ influx into roots（α）, thereby promoted the absorption of Zn. Inoculation of Funneliformis mosseae reduced the Km value but inoculation of Rhizophagus intraradices increased the Km value, suggesting that there might bear a certain relationship with the difference of the effects of different AM fungi on the root morphology of winter wheat and Zn transporter gene expression in winter wheat roots.

关 键 词：丛枝菌根真菌 吸收动力学 侵染率 根系形态 

分 类 号：S512.11[农业科学—作物学]