AM真菌与镉互作影响桑生长和无机元素吸收转运  

Effects of interaction between AMF and Cd on mulberry growth and absorption and migration of inorganic elements

作　　者：王凯渊 蒋园园 宋文俊 刘家艳 黄先智[3] 杨晓红 

机构地区：[1]西南大学园艺园林学院南方山地园艺学教育部重点实验室,重庆400716 [2]重庆市南山植物园管理处,重庆400065 [3]西南大学蚕学与系统生物学研究所,重庆400716

出　　处：《菌物学报》2017年第7期996-1009,共14页Mycosystema

基　　金：现代农业产业技术体系专项(CARS-22-ZJ0503);重庆市研究生教育教学改革研究项目(yjg143028)~~

摘　　要：为评价Cd胁迫下接种AM真菌(AMF)对减轻桑树Cd毒害及Cd迁移规律的影响,采用了分室培养法开展试验。在菌根室种植无菌桑苗并接种AMF(Gigaspora rosea),菌丝室设置兼菌丝收集器功能的Cd陷阱。陷阱中的Cd处理浓度为0、5、20、40mg/kg,菌根室每室种植3株桑苗,6个重复,对照不接种AMF。培养60d后检测桑苗的菌根侵染率、不同部位的生长量、AMF菌丝生物量、4种矿质营养元素组分及Cd在AMF菌丝体和桑株中的定位及转运情况。结果表明:在0–40mg/kg的Cd处理下,不接种AMF处理的对照桑,其菌根侵染率、AMF菌丝生物量以及镉含量都为0。接种AMF处理中,当Cd<5mg/kg时,桑苗菌根侵染率、AMF菌丝生物量、桑苗鲜生物量、枝叶中N、K、Ca、Mg以及根系N、K、Ca矿质元素的积累都有促进作用,在5mg/kg时促生效果最佳;当Cd>5mg/kg时,随着Cd浓度的升高,桑苗菌根侵染率、AMF菌丝生物量、桑苗鲜生物量、枝叶中N、K、Ca、Mg以及根系N、K、Ca矿质元素的积累都受到渐强的抑制;陷阱中Cd浓度高低与根系中Mg元素的吸收量之间显著负相关;接种AMF可以显著提高桑株生物量和矿质营养的积累;Cd主要集中在桑苗的根部,Cd处理浓度与AMF菌丝体中以及桑株中的Cd含量均显著正相关;Cd在菌根桑苗中的迁移率(<9%)和不同部位(根-茎、根-叶)的转移系数(<0.1)都极其低。结果证明:Cd胁迫对桑苗的菌根侵染率、营养生长、AMF菌丝生长和矿质吸收(除Mg外)都存在低促高抑的剂量效应;AMF对Cd胁迫有一定的抗性,接种AMF显著促进植物的生长以及矿质营养元素的吸收和转运;重金属Cd在菌根桑中的分布、迁移是不均匀的,具有一定的独特性。Compartment culture experiment was carried out for evaluating the effect of AMF on the Cd poisoning and Cd migration in mulberry under Cd stress. Aseptic mulberry saplings were planted in mycorrhizal compartment and inoculated with AMF spore of Gigaspora rosea. Cd trap was set up, serving simultaneously the function of mycelial collector. The Cd treatment concentration was 0, 5, 20 and 40mg/kg in the trap. Three mulberry seedlings were planted in each mycorrhizal compartment. Six replicates were set up. The control was not inoculated with AMF. The mycorrhizal infection rate, the growth biomass and mineral nutrient uptake of seedlings, AMF mycelial biomass in compartment, Cd content in AMF mycelia, as well as Cd location and migration in different position of mulberry saplings, were checked after being cultured for 60 days respectively. The results showed that mycorrhizal infection rate, the AMF mycelial biomass and Cd content were not detected in mulberry uninoculated with AMF under Cd treatment concentration of 0–40mg/kg. Inoculation with AMF, mycorrhizal infection rate, mulberry seedling biomass, accumulation of N, K, Ca, Mg in shoots, accumulation of N, K, Ca in roots and AMF biomass were improved markedly. The best stimulated growth effect was achieved at 5mg/kg concentration of Cd. When Cd concentration was 5mg/kg, mycorrhizal infection rate, mulberry seedling biomass, accumulation of N, K, Ca, Mg in shoots, accumulation of N, K, Ca in roots and AMF mycelial biomass were gradually inhibited with the increase of Cd concentration. There was a significant negative correlation between Cd concentration in the trap and Mg absorption of roots. Inoculation of AMF can significantly improve biomass and mineral nutrient accumulation of mulberry seedlings. Cd was mainly concentrated in mulberry roots. The Cd treating concentration significantly positively correlated with the Cd content of mycelium in trap and mulberry plants. The transfer rate（9%） and transfer coefficient（0.1） of Cd in mycorrhizal mulberry sap

关 键 词：丛枝菌根真菌(AMF) 玫瑰红巨孢球囊霉 桑树 Cd迁移 剂量效应 

分 类 号：S888.2[农业科学—特种经济动物饲养]