萋-尼染色导致的总挥发性有机物污染及其控制方法  

Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures

作　　者：金浩龙[1] 陈志谨[2] 熊昆[2] 金晓琳[2] 

机构地区：[1]第三军医大学新桥医院感染控制科,重庆400037 [2]第三军医大学基础医学部微生物学教研室重庆市微生物工程实验室,重庆400038

出　　处：《第三军医大学学报》2017年第14期1481-1485,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(31570173)~~

摘　　要：目的建立一种改良抗酸染色冷染方法,用以控制实验室总挥发性有机物(total volatile organic compound,TVOC)污染。方法采用TVOC检测仪定量测定实验室内空气中的苯系物和TVOC污染程度;以卡介苗(bacillecalmette-guerin,BCG)为染色标本,配制含有1%Triton X-100标本稀释液和初染液,建立不用加温助染的抗酸染色冷染法替代传统抗酸染色法。结果传统萋-尼抗酸染色时,室内TVOC污染在12 min时间点即高达1 014 ppb(约1 mg/m^3),成倍超过国家室内卫生标准(GB50325-2010)规定值(500 ppb,即0.5 mg/m^3);同样条件下,用改良冷染法染色期间,室内TVOC量有所增加,但在试验期间最高值为387 ppb,保持在国家室内卫生标准容许限度以下;利用Triton X-100建立的改良抗酸冷染法具有抗酸染色特性,其对抗酸杆菌的可检测度为3×10^(-6)~6×10^(-5)CFU/mL。结论抗酸染色冷染法可用于替代传统萋-尼抗酸染色,从而控制实验室的TVOC污染问题。Objective To detect total volatile organic compound（ TVOC） pollution in the laboratory during Ziehl-Neelsen acid-fast staining and establish an improved method for controlling TVOC pollution.Methods TVOC concentration in the room air of the laboratory was determined using RAE portable-type instrument during the performance of routine acid-fast staining of Bacille Calmette-Guerin（ BCG）. A dilution solution and a carbolfuchsin solution containing 1% Triton X-100 were prepared for use in the modified cold acid-fast staining without heating,and the TVOC pollution level caused by the modified method and the routine acid-fast staining method was compared. Results At 12 min after the start of routine acid-fast staining,the concentration of TVOC in the room air of the laboratory reached the highest level of 1 014 ppb（ about 1 mg/m^3）,which was 2 folds of the permissible exposure limit（ 500 ppb,0. 5 mg/m^3） in the National Standard（ GB50325-2010）. During the performance of the modified cold acid-fast staining,TVOC concentration in the laboratory increased only slightly and remained under the permissible exposure limit. The detection sensitivity of BCG ranged from 3 × 10^-6 to 6 × 10^-5 CFU using the modified cold staining method.Conclusion The modified acid-fast cold staining method can be used as an alternative to the conventional acid-fast staining for controlling TVOC pollution in the laboratory.

关 键 词：萋-尼染色 TVOC污染 冷染色法 

分 类 号：R136.32[医药卫生—劳动卫生] R446[医药卫生—公共卫生与预防医学]