Roux-en-Y胃旁路术对2型糖尿病大鼠肝及外周胰岛素敏感性的影响  

Roux-en-Y gastric bypass increases hepatic and peripheral insulin sensitivity in rats with type 2 diabetes mellitus

作　　者：刘红媛[1] 马南希 马瑞[1] 何冰[1] 

机构地区：[1]中国医科大学附属盛京医院内分泌科,沈阳110004

出　　处：《中国实验动物学报》2017年第3期270-274,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金面上项目(NO.81570765);辽宁省科学技术基金(NO.2015020539)

摘　　要：目的观察RYGB术后不同时间2型糖尿病大鼠肝及外周组织胰岛素敏感性的变化并初步探讨可能机制。方法 RYGB组(n=21)及RYGB假手术组(n=7),采用清醒状态扩展高胰岛素-正血糖钳夹术,评估RYGB术后2、4、8周肝及外周组织胰岛素敏感性。检测肝及腓肠肌内TG水平。结果 RYGB术降低体重,减少体内脂肪,改善脂代谢。术后2周肝胰岛素敏感性指数显著提高,肝TG含量明显下降(P<0.05)。术后4周M值显著提高,肌肉TG含量明显下降(P<0.05)。结论 RYGB术后肝胰岛素敏感性的提高早于外周组织,肝及外周组织胰岛素抵抗持续改善有助于2型糖尿病的长期缓解。RYGB术减少肝及骨骼肌脂肪沉积可能是术后胰岛素敏感性上调的重要机制之一。Objective To investigate the effects of Roux-en-Y gastric bypass （RYGB） on hepatic and peripheral insulin sensitivity in rats with type 2 diabetes and their possible mechanisms.Methods 5-6 week old SPF male Sprague-Dawley rats were divided into 2 groups： diabetic RYGB group （n=21） and diabetic RYGB sham group （n=7）.The hyperinsulinemic-euglycemic clamp with tracer infusion was completed at 2,4,and 8 weeks postoperatively to assess the insulin sensitivity.The lipid content in liver and muscle tissue was examined.Results Postoperatively,the diabetic RYGB group had significant decreases in weight,fat mass,and RYGB had a profound effect on the plasma lipid profile.Two weeks after surgery,the hepatic insulin sensitivity index was significantly improved and the hepatic triglyceride was decreased in the RYGB group （P〈0.05）.The significant increased insulin sensitivity was not detected until four weeks after RYGB surgery,the M value was significantly increased and the TG content in the muscle tissue was significantly decreased （P〈0.05）.Conclusions The increased insulin sensitivity after RYGB occurs earlier in the liver than in the muscle and both may contribute to the long-term remission of type 2 diabetes.Reduced lipid content in hepatocytes and skeletal muscle cells after RYGB may contribute to the improved insulin sensitivity in these cells.

关 键 词：2型糖尿病 肥胖 胰岛素抵抗 ROUX 胰胃旁路术 

分 类 号：Q95-33[生物学—动物学]