改善Ⅱ型糖尿病大鼠糖代谢运动模型的建立  

Establishment of an improved exercise-induced rat model of glycometabolism of type Ⅱ diabetes

作　　者：上官若男[1,2] 朱斌[2] 尚画雨 苏全生[3] 

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]成都大学体育学院,成都610106 [3]成都体育学院,成都610041

出　　处：《中国实验动物学报》2017年第3期275-280,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：四川省教育厅人文社会科学重点研究基地-四川休闲体育产业发展研究中心课题资助(编号:XXTYCY2015C03);成都大学校青年自然科学基金资助(编号:2017XJZ16)

摘　　要：目的建立运动改善Ⅱ型糖尿病大鼠糖代谢模型,为Ⅱ型糖尿病运动处方的建立提供理论参考。方法 SPF级雄性8周龄Wistar大鼠45只,随机抽取32只在高糖高脂饲料喂养7周的基础上腹腔注射小剂量链脲佐菌素(STZ)缓冲液,建立Ⅱ型糖尿病大鼠模型。将正常大鼠和造模成功的大鼠分为4组:空白对照组(C组)、单纯运动组(CE组)、糖尿病对照组(DM组)、糖尿病运动组(DME组)。运动组采用改进的Ploug训练方案,60min/d,每周训练6 d,共训练8周。分别在高脂饲料喂养7周后尾静脉取血测定空腹血糖和血清胰岛素,造模后基线时间和运动8周末尾静脉取血测定空腹血糖(FBG),运动8周末眶后取血测定血清胰岛素(FINS),并计算胰岛素抵抗指数(HOMA-IR)。结果 (1)7周高糖高脂喂养后,与正常组相比,高脂组FBG、FINS和HOMA-IR含量显著升高。(2)8周运动干预后,DM组和DME组FINS水平显著低于C组和CE组,FBG和HOMA-IR显著高于C组和CE组;DME组FINS水平显著高于DM组,FBG和HOMA-IR显著低于DM组。DM组和DME组体重显著低于C组和CE组;DME组与DM组、CE组与C组组间体重差异无显著性。结论 (1)7周高糖高脂喂养联合一次性腹腔注射STZ(30 mg/kg)成功建立Ⅱ型糖尿病大鼠模型;(2)60 min/d共8周的游泳运动模式能够改善Ⅱ型糖尿病大鼠糖代谢,是研究运动锻炼预防和改善糖尿病机理的理想动物模型。Objective To establish an improved model of exercise-induced glycometabolism in type II diabetic rats,and to provide a theoretical reference for the establishment of exercise prescription for type II diabetes.Methods Forty-five 8-week old SPF male Wistar rats were used in this study.Of which 32 were fed with high-fat diet for 7 weeks,and intraperitoneal injection of 30 mg/kg STZ was given to establish the rat model of type II diabetes.The normal rats and successful model rats were divided into four groups：The normal control group （C group）,normal exercise group （CE group）,diabetic group （DM group） and diabetic exercise group （DME group）.The exercise group was assigned by the Ploug training protocol,6 days/week,60 min/day,for a total of 8 weeks.After the high fat diet fed for 7 weeks,blood sample was taken from the tail vein,FBG and serum insulin were detected after baseline and 8 weeks exercise,and blood sample was collected from the tail vein to determine the FBG.Serum insulin （FINS） was detected by orbital blood sampling at the end of 8 weeks of exercise,and HOMA-IR was calculated.Results 1.After 7 weeks of high fat diet,compared with the groups C and CE,the levels of FBG,FINS and HOMA-IR were significantly higher in the DM and DME groups.2.After 8 weeks of exercise intervention,compared with the groups C and CE,FINS was significantly lower in the groups DM and DME,but the FBG and HOMA-IR were higher.Compared with the DM group,the level of FINS was significantly higher in the DME group,and the levels of FBG and HOMA-IR were significantly lower.The body weights of DM and DME groups were significantly lower than those of the groups Cand CE,the body weight had no significant difference between the DME and DM groups,and similar result was between the groups CE and C.Conclusions 1.The rat model of type II diabetes is successfully established with high fat diet for 7 weeks plus STZ injection（30 mg/mL）.2.Aerobic exercise 60 min/day for a total of 8 weeks can improve the glycometabolism in typ

关 键 词：游泳运动 Ⅱ型糖尿病 高糖高脂饮食 运动大鼠模型 糖代谢 

分 类 号：Q95-33[生物学—动物学]