功能性消化不良脾虚证大鼠模型的建立及评价  

Establishment and assessment of a rat model of functional dyspepsia with spleen deficiency

作　　者：刘成全 邓青 谭志超 杨华 姜德建 曾贵荣[1,3] 

机构地区：[1]湖南省药物安全评价研究中心新药药效与安全性评价湖南省重点实验室,长沙410331 [2]湖南汉森医药研究有限公司,长沙410013 [3]北京协和医学院药用植物研究所,北京100001

出　　处：《中国实验动物学报》2017年第3期311-315,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：湖湘青年创新人才基金

摘　　要：目的探讨功能性消化不良脾虚证动物模型的建立及比较。方法分别采用碘乙酰以及复合小平台站立或复合游泳法建立功能性消化不良脾虚证大鼠模型,通过观察动物一般状态、体重及摄食量变化,检测血清胃动素、胆囊收缩素、乳酸、胃泌素的含量、尿D-木糖排泄率来评价成模效果。结果碘乙酰胺单因素组大鼠体重增长减缓、摄食量减少;复合因素组大鼠出现消瘦、毛发枯涩、腹泻等脾虚症状,且大鼠尿D-木糖排泄率、血清胃动素、胃泌素含量明显降低,而血清胆囊收缩素、乳酸含量明显升高。结论三种造模方法均能导致功能性消化不良脾虚症状,但复合因素处理组脾虚症状更明显,碘乙酰胺复合小平台站立法成模效果最佳。Objective To establish animal models of functional dyspepsia with spleen deficiency and to compare the efficacy of different methods.Methods Rat models were established by iodoacetamide（IA）-treatment or combined with swimming.Appearance,body weight,food intake of the rats were observed,and serum motilin,cholecystokinin,lactate,gastrin content and urinary D-xylose excretion rates were detected to confirm whether the model of functional dyspepsia with spleen deficiency was established.Results The IA-treated rats had less food intake and a slower body weight gain.The IA-treated combined with swimming rats presented spleen-hypofunction symptoms,such as emaciation,hair dry and loose stools,their urinary D-xylose excretion rate,serum motilin,gastrin content were decreased,and serum cholecystokinin and lactate contents were increased significantly （P〈0.05 for all）.Conclusions All the three methods used in this study can result in symptoms of functional dyspepsia with spleen deficiency.However,IA-treatment combined with swimming models appear more close to spleen deficiency-like presentation,and the best model is the IA-treated combined with platform standing.

关 键 词：功能性消化不良 脾虚症 碘乙酰胺 大鼠 

分 类 号：Q95-33[生物学—动物学]