反相高效液相色谱法检测斑蝥黄的含量  

RP-HPLC determination of content of Canthaxanthin

作　　者：吴凤银 

机构地区：[1]广州巨元生化有限公司,广东广州510000

出　　处：《广东化工》2017年第13期259-260,共2页Guangdong Chemical Industry

摘　　要：目的:建立反相高效液相色谱法测定斑蝥黄的含量。方法:采用日本岛津VP-ODS C18柱(150 mm×4.6 mm,5μm);流动相:甲醇-二氯甲烷=85-15;流速:1.0 m L/min;检测波长:450 nm;柱温:30℃。结果:斑蝥黄异构体能较好的分离,斑蝥黄在0.7μg^70μg/m L范围内呈现良好的线性关系(r=0.9998);重复性试验测得的精密度RSD=0.11%(n=5),平均回收率为99.6%(RSD=0.30%,n=9)。结论:该方法操作简便、灵敏、准确,能够满足检测斑蝥黄含量的要求。Objective： To establish an RP-HPLC method for determination of canthaxanthin content. Method： Japan Shimadzu VP-ODS C18 column （150 mm × 4.6 mm, 5 μm） was adopted with the mobile phase methanol ： dichloromethane=85 ： 15 at the flow rate of 1.0 mL/min; detection wavelength was at 450 nm, and the column temperature was 30 ℃. Results： The main peak was well separated from adjacent impurities and the linear range of the method was 0.7 lag-70 μg/mL （r=0.9998）; RSD of repetitive test was 0.11% （n=5）; the average recovery was 99.6 % （n=9）. Conclusion： The established method was sensitive, accurate and specific, which can be used for canthaxanthin determination.

关 键 词：反相高效液相色谱法 斑蝥黄 含量测定 

分 类 号：O65[理学—分析化学]