SGBG/PHBV与人牙周膜细胞的生物相容性  

Biocompatibility between SGBG/PHBV and Human PDLC

作　　者：黄南楠[1] 孙文娟[1] 吴坚[1] 李婵秀[1] 唐倩[1] 

机构地区：[1]中山大学附属第三医院口腔科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2017年第4期526-531,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2010B031100021)

摘　　要：【目的】研究溶胶-凝胶生物活性玻璃(SGBG)/聚羟基丁酸戊酸共聚酯(PHBV)与人牙周韧带细胞(PDLC)的生物相容性,为牙周组织工程支架的选择提供依据。【方法】采用组织块法培养原代人PDLC,分为浸提液组和对照组接种于96孔板,每组9孔,分别加入浓度为100%、75%、50%、25%、0%的浸提液,48 h后采用MTT比色分析法的浸提液实验对材料进行毒性评级。采用人PDLC与SGBG/PHBV体外三维培养,扫描电镜下观察细胞形态及生长情况。实验组采用SGBG/PHBV复合培养,对照组采用普通培养基培养,培养12、24、48 h后,每组取27个样本定量检测上清液碱性磷酸酶(ALP)含量。【结果】浸提液培养结果显示:毒性评级为1级和0级。扫描电镜示人PDLC在三维支架上贴附生长均良好。实验组上清液ALP含量高于对照组,差异有统计学意义(P<0.05)。【结论】SGBG/PHBV对人PDLC无毒性,SGBG/PHBV和人PDLC三维培养,表现出良好的生物相容性,有望应用于进一步的牙周组织工程的研究。[Objective] The biocompatibility between SGBG/PHBV and human PDLC were investigated to provide a basis for the choice of the scarf material of periodontal tissue engineering. [ Methods ] Human PDLC were cultured using tissue-explant technique. Seeding on 96 wells plate, 9 wells for one group , Four different concentrations （ 100% , 75% , 50% , 25% , 0% ） of maceration extract of SGBG/PHBV were added into the culture plantsafter 48-h cell seeding, the grades of the cytotoxicity of SGBG/PHBV was evaluated by MTI＂ assay. Human PDLC cultured in vitro were collected and seeded on the three-dimensional scaffolds of SGBG/PH- BV, the cellular morphology and cell growth on the scaffolds were observed and photographed by scanning electronic microscope. HumanPDLC seeded on the three-dimensional scaffolds of SGBG/PHBV in the experimental group, and human PDLC seeded by DMEM in the control group, after 12-, 24-, and 48-h cell seeding, got 27 simples for each group, and the affection of the SGBG/ PHBV on cell secretory function was observed by spectrophotometry which assayed the biochemical indexes ALP in supinate. [ Results ] The grades of the cytotoxicity of SGBG/PHBV were 0 and 1. It was displayed that human PDLC adhered and proliferat- ed well on the scaffold of SGBG/PHBV under the scanning electronic microscope. The significant difference of ALP in supinate be- tween the experimental group and the control group （P 〈 0.05）. [Conclusions] SGBG/PHBV had no cytotoxicity to human PDLC. SGBG/PHBV is potential to further study as the scaffolds of periodontal tissue engineering since it displayed the satisfactory biocom- patibility with human PDLC.

关 键 词：溶胶-凝胶生物活性玻璃/聚羟基丁酸戊酸共聚酯 人牙周韧带细胞 牙周组织工程 

分 类 号：R318[医药卫生—生物医学工程]