中药复方熄风胶囊对难治性癫痫大鼠多药耐药基因MDR1mRNA表达的影响  

Effect of the Chinese herbal compound Xifeng capsule on expression of MDR1mRNA of intractable epilepsy rats

作　　者：陈会[1] 李新民[1] 路岩莉[1] 孙丹[1] 聂坤[1] 房艳艳[2] 任艳艳 

机构地区：[1]天津中医药大学第一附属医院儿科,300193 [2]天津中医药大学研究生院 [3]山东省邹城市妇幼保健计划生育服务中心

出　　处：《环球中医药》2017年第7期792-796,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81373690)

摘　　要：目的探讨中药复方熄风胶囊对氯化锂—匹罗卡品致难治性癫痫模型大鼠脑组织MDR1mRNA表达的影响。方法建立氯化锂—匹罗卡品癫痫大鼠模型。实验大鼠随机分为8组:正常对照组(空白组)、模型对照组(模型组)、熄风胶囊低剂量组(熄低组)、熄风胶囊中剂量组(熄中组)、熄风胶囊高剂量组(熄高组)、卡马西平治疗组(CBZ组)、熄风胶囊中剂量+卡马西平组(熄卡组)、熄风胶囊中剂量+1/2卡马西平组(熄卡低组)。熄低、中、高组分别予熄风胶囊0.33 g、0.66 g、0.99 g,浓缩剂2 m L;CBZ组予CBZ 20 mg/kg;熄卡、熄卡低组分别予熄风胶囊0.66 g和CBZ20 mg/kg、CBZ 10 mg/kg;模型组和空白组分别予生理盐水2 m L。每天上午灌胃一次,共持续60天。给药结束后检测各组大鼠脑组织MDR1mRNA的表达。结果与空白组比较,其余各组的MDR1mRNA的基因表达均上调(P<0.05);与模型组比较,熄中组、熄卡低组、熄卡组和CBZ组的MDR1mRNA表达均下降(P<0.05);与CBZ组相比,熄卡低组和熄卡组的基因表达均明显降低(P<0.05)。结论熄中组、熄卡低组、熄卡组、CBZ组对MDR1mRNA表达有抑制作用,且熄卡低组和熄卡组对MDR1mRNA表达的抑制作用比单用卡马西平作用更明显。Objective To study the effect of Chinese herbal compound Xifeng capsule on different expression of MDR1mRNA in the brain tissue of epileptic rats induced by lithium chloride-pilocar.Methods Epileptic rat model was established by lithium chloride-pilocarpine.Rats were randomly divided into eight groups： normal control group, model control group, Xifeng capsule low-dose treatment group （Xifeng low-dose group）, Xifeng capsule middle-dose treatment group （Xifeng middle-dose group）, Xifeng capsule high-dose treatment group （Xifeng high-dose group）, carbamazepine treatment group（CBZ group）, Xifeng middle-dose ＋ carbamazepine treatment group （xi-car group）, Xifeng capsule middle-dose＋1/2dose carbamazepine treatment （xi-car low group）.Xifeng low, medium and high group was respectively given 0.33 g, 0.66 g and 0.99 g, thickening agent 2 mL;CBZ dose group was given CBZ 20 mg/kg;Xi-car and Xi-car low group was respectively given Xifeng capsule 0.66g thickening agentr 2mL and CBZ 20mg/kg, CBZ 10mg/kg;normal control group and model control group was respectively given 2 mL saline solution.The rats were intragastric administration once a day in the morning for 60 days.The MDR1mRNA expression of each group was detected.Result Real-time RT-PCR results showed that： comparing with normal control group, the expression of MDR1mRNA in the rest groups were up-regulation.Comparing with model group, the expression of MDR1mRNA of the CBZ group,Xifeng middle-dosegroup,Xifeng middle-dose＋CBZ groupand Xifeng middle-dose＋1/2 CBZ group was declined（P〈0.05）.Comparing with CBZ group,the expression of MDR1mRNA of Xifeng middle-dose＋CBZ groupand Xifeng middle-dose＋1/2 CBZ group was declined（P〈0.05）.Conclusion The CBZ group,Xifeng middle-dosegroup,Xifeng middle-dose＋CBZ group and Xifeng middle-dose＋1/2 CBZ group had inhibitory effect on the expression of MDR1mRNA, and Xifeng middle-dose group,the inhibitory effect of Xifeng middle-dose＋1/2 CBZ group is more obvious than CBZ group.

关 键 词：中药复方 难治性癫痫 多药耐药基因 熄风胶囊 

分 类 号：R285.5[医药卫生—中药学]