化浊解毒活血通络方对脑缺血再灌注损伤大鼠ZO-1和occludin表达的影响  

Effects of Huazhuo Jiedu Huoxue Tongluo prescription on expression of tight junction ZO-1 and occludin in rats with cerebral ischemia reperfusion injury

作　　者：李希[1] 万溪 高晶晶[1] 赵层闪 刘学飞[3] 杨丽静[3] 徐丽娟[3] 田军彪[1,3] 

机构地区：[1]河北医科大学研究生院 [2]河北省衡水市中医院 [3]河北省中医院神经内科

出　　处：《环球中医药》2017年第7期796-801,共6页Global Traditional Chinese Medicine

基　　金：河北省自然科学基金面上项目(H2013206393);河北省科技支撑计划(15277748D);河北省省级中医药类重大医学科研课题(zyzd2013004);河北省中医药管理局科研计划(2014039)

摘　　要：目的观察化浊解毒活血通络方对脑缺血再灌注损伤模型大鼠紧密连接蛋白ZO-1和occludin表达的影响,探讨其神经保护机制。方法以改进后的Zea-Longa线栓法制备局部梗死性脑缺血再灌注损伤模型,以Longa评分法进行神经功能缺损评分,光镜下观察脑组织形态改变,免疫组化法观察ZO-1和occludin的分布及表达,RT-PCR法评测其在各组大鼠缺血侧脑组织中表达量。结果免疫组化结果显示,与假手术组相比,模型组ZO-1表达水平明显降低(P<0.05);与模型组相比,各用药组ZO-1的表达水平均有升高(P<0.05),其中以尼莫地平组和中药高剂量组最为明显。与假手术中相比,模型组occludin表达明显减弱(P<0.05),与模型组相比,各用药组occludin表达均有增加(P<0.05);中药低剂量组、中剂量组、高剂量组表达依次增加(P<0.05),高剂量组与尼莫地平组差别不明显(P>0.05)。RT-PCR法显示:与假手术组相比,模型组ZO-1及occludin表达水平明显降低(P<0.05);与模型组相比,各用药组ZO-1及occludin的表达水平均有升高(P<0.05),其中以尼莫地平组和高剂量组最为显著。结论化浊解毒活血通络方可能通过增加ZO-1和occludin的表达、调节血脑屏障通透性等机制,发挥神经保护作用。Objective To investigate the effects of Huazhuo Jiedu Huoxue Tongluo prescription on expression of tight junction protein ZO-1 and occludin in rats with Cerebral ischemia reperfusion Injury, and explore the neuroprotective mechanism.Methods The rat model of cerebral ischemia-reperfusion injury（CIRI） was induced by Zea-Longa method.The neurological severity score was assessed by longa grade point standard, the change of ischemic brain tissue was observed under light microscope, and the expression of ZO-1 and occludin was detected by immunohistochemistry and RT-PCR method.Results The immunohistochemical results showed that compared with sham operation group, the expression of ZO-1 in model group was lower （P〈0.05）.Compared with the model group, the expression of ZO-1 in drugs group was obviously higher （P〈0.05）, the nimodipine group and high dose group was increased most obviously（P〈0.05）.Compared with sham operation group, the expression of occludin in model group was lower （P〈0.05）.Compared with model group, the expression of drugs group was obviously higher （P〈0.05）, the expression of middle dose group was higher than that in the low dose group（P〈0.05）, lower than that in the high dose group（P〈0.05）.There was no significant difference between nimodipine group and high dose group （P〉0.05）.The RT-PCR method showed that compared with sham operation group, the expression of ZO-1 and occludin in model group were lower （P〈0.05）.Compared with the model group, the expression of ZO-1 and occludin in drugs group was obviously higher （P〈0.05）, the nimodipine group and high dosegroup were increased most obviously（P〈0.05）.Conclusion Huazhuo Jiedu Huoxue Tongluo prescription may play a neuroprotective role by increasing the expression of ZO-1 and occludin and regulating the permeability of blood-brain barrier.

关 键 词：化浊解毒活血通络方 脑缺血再灌注损伤 紧密连接蛋白 

分 类 号：R285.5[医药卫生—中药学]