青娥丸对去卵巢大鼠骨质疏松骨微循环的作用机制研究  

Effect and mechanism of Qing'E formula on bone microcirculation of osteoporosis rats induced by ovariectomy

作　　者：王晓燕[1] 常时新[2] 李冠武[2] 何静[2] 王乐[2] 宋彦颖[2] 缪宇[2] 

机构地区：[1]中国中医科学院广安门医院肿瘤科,北京100053 [2]上海中医药大学附属岳阳中西医结合医院放射科

出　　处：《环球中医药》2017年第7期802-807,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81373856)

摘　　要：目的通过观察青娥丸对卵巢切除骨质疏松大鼠骨髓灌注及血管内皮生长因子的影响,探讨经典方剂青娥丸对绝经后骨质疏松骨微循环的作用机制。方法将6月龄SD雌性大鼠60只随机分为4组,即假手术组(SHAM组)、去势对照组(OVX组)、青娥丸组(QEW组)和雌激素组(E_2组)。建模8周后,分别进行药物干预3个月,检测各组大鼠股骨远端、中端、近端的骨髓灌注参数以及血管内皮标志物血小板-内皮细胞黏附分子(platelet endothelial cell adhesion molecule-1,CD31)、血管内皮生长因子(vascular endothelium growth factor,VEGF)表达情况。结果骨髓灌注参数:(1)股骨远端:治疗后OVX组容积转移常量(Ktrans)、速率常数(Kep)、渗漏空间(Ve)水平较治疗前均有明显降低(P<0.01),E_2组和QEW组Ktrans、Kep、Ve水平较治疗前均有明显增加(P<0.05);与OVX组比较,治疗后E_2组和QEW组Ktrans、Kep、Ve水平均显著增加(P<0.01);而QEW组Ktrans水平显著低于E_2组(P<0.01)、两组Kep、Ve水平无明显统计学差异。(2)股骨中端:治疗后,OVX组Ktrans(P<0.01)及Ve(P<0.05)水平较治疗前显著降低,E_2组Ktrans、Kep及Ve水平均明显增加(P<0.05);与OVX组比较,E_2组Ktrans、Kep(P<0.05)及Ve(P<0.01)水平均显著增加,QWE组Ktrans(P<0.05)、Ve(P<0.01)水平显著增加。(3)股骨近端:E_2组Ktrans(P<0.01)及Ve(P<0.05)水平较OVX组均显著增加,而QEW组Ktrans水平无明显差异。血管内皮标志物CD31、VEGF表达情况:E_2组和QEW组CD31及VEGF蛋白表达及相对表达量较OVX组均显著上调(P<0.05、P<0.01)。结论青娥丸治疗绝经后大鼠骨质疏松症,可能与提高骨髓灌注、改善骨髓微循环等作用机制相关。Objective To observe the effects of Qing＆#39;＆#39;E formula on bone marrow perfusion and vascular endothelial grouth factor in Postmenopausal Osteoporosis（PMOP） rats induced by oophorectomy, explore the possible mechanism of classical prescription Qing＆#39;＆#39;E formula on bone microcirculation.Methods Sixty female SD rats of 6 month-old infants were randomly divided into SHAM group, OVX group, Qing＆#39;＆#39;E formula group and E2 group, PMOP model was established by oophorectomy, 8 weeks after modeling, rats were medicated once a day for 3 months.Detect the bone marrow perfusion parameters of distal femur, mid femur and proximal femur, as well as vascular endothelial marker expression.Results Bone marrow perfusion parameters： （1） Distal femur： The level of Ktrans, Kep, Ve in OVX group were significantly decreased while those were increased in E2 group and QEW group after treatment, and significantly higher than OVX group.The level of Ktrans in QEW group were significantly lower than E2 group, the level of Kep and Ve have no significant difference.（2） Mid femur： the level of Ktrans and Ve in OVX group were significantly decreased.The level of Ktrans, Kep and Ve in E2 group were significantly increased and Ktrans and Ve increased in QEW group were significantly higher than OVX group.（3） Proximal femur： the level of Ktrans and Ve in E2 group were significantly higher than OVX group, but no significant differences between two groups.Vascular endothelial marker CD31, VEGF expression： the protein expression and relative expression of CD31 and VEGF in E2 group and QEW group were significantly increased compared with OVX group.Conclusion Qing＆#39;＆#39;E formula can treat PMOP, which may be associated with increasing bone marrow perfusion and improving bone marrow microcirculation.

关 键 词：绝经后骨质疏松症 青娥丸 骨髓灌注 骨髓微循环 

分 类 号：R285.5[医药卫生—中药学]