超声波激活氧氟沙星声动力损伤牛血清白蛋白的研究  

Sonodynamic damage of bovine serum albumin under ultrasonic irradiation in the presence of ofloxacin

作　　者：高博[1] 王新[1] 刘彬[1] 景姣姣 左子凡 王景成 牛华英 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036 [2]济南大成医药发展有限公司,山东济南250100

出　　处：《药学研究》2017年第7期373-375,395,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.21402125)

摘　　要：目的考察Fe^(3+)、Zn^(2+)金属离子(M^(n+))对于超声波激活氧氟沙星(OFX)损伤牛血清白蛋白(BSA)的影响。方法采用紫外-可见光谱和荧光光谱,探讨了超声照射时间和Fe^(3+)、Zn^(2+)金属离子浓度等因素对牛血清白蛋白损伤的影响。结果在一定条件下,牛血清白蛋白的损伤程度随超声波照射时间的延长和Fe^(3+)、Zn^(2+)浓度的增大而加剧,并且Fe^(3+)对于超声激活氧氟沙星损伤牛血清白蛋白的促进作用明显强于Zn^(2+)。结论 Fe^(3+)可明显增强超声波激活氧氟沙星对牛血清白蛋白的损伤。Objective To study the effects of Fe^（3＋）and Zn^（2＋）on the damage of bovine serum albumin（ BSA） molecules in the presence of ofloxacin（ OFX） activated by ultrasonic irradiation.Methods The influencing factors,such as ultrasonic irradiation time and metal ion concentration,were investigated by UV-Vis spectroscopy and fluorescence spectroscopy. Results The damage of BSA was enhanced with the increasing of the ultrasonic irradiation time and metal ion concentration.The enhance effect of Fe^（3＋）on sonodynamic damage of BSA from OFX activated by ultrasound was much stronger than that of Zn^（2＋）.Conclusion Fe^（3＋）can enhance the damage of BSA in the presence of OFX activated by ultrasonic irradiation.

关 键 词：超声波 氧氟沙星 牛血清白蛋白 损伤 

分 类 号：O644.3[理学—物理化学]