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作 者:路鹏鹏[1] 孙晓宇[1] 陈锐[1] 瞿佳[1] 赵玲侠[1] 韩丽萍[1] 沈卫荣[1] LU Peng-peng SUN Xiao-yu CHEN Rui QU Jia ZHAO Ling-xia HAN Li-ping SHEN Wei-rong(Microbiology Institute ofShaanxi, Xi' an 710043, China)
出 处:《广东农业科学》2017年第4期113-117,共5页Guangdong Agricultural Sciences
基 金:陕西省科学院重大专项(2012k-03);陕西省科学院青年人才培养专项(2014k-30);陕西省农业科技创新项目(2012NKC02-14);陕西农业科技创新与攻关项目(2015NY088)
摘 要:以室内生物测定检测枝顶孢霉悬液对南方根结线虫二龄幼虫的致死率,在黄瓜砧木上,以盆栽试验和温室小区试验检测枝顶孢霉粉剂对南方根结线虫病的防效。结果表明,室内生物测定枝顶孢霉悬液对南方根结线虫的最高致死率为89.7%,毒力曲线为y =-7.5989+1.717826x,R^2=0.9960,LC_(50)=2.15×10~7 CFU/ mL;盆栽试验最佳防效为药后14 d,防效为15.38%~31.57%,各处理有剂量-效应关系,对黄瓜砧木生长有促进作用。温室小区最佳防效为药后120 d,防效为80.47%~84.38%。枝顶孢霉粉剂对南方根结线虫有一定的防效,同时可以促进黄瓜砧木的生长,需要在早期病害较轻时施用,药后4个月左右需要补施一次。The lethality of secondary stage juvenile of Meloidogyne incongnita was detected with the variation of suspension concentration of Acremonium sp. by indoor bioassay,and the control efficiency of Acremonium sp. powder against M. incongnita was detected by pot experiment and greenhouse area test in cucumber root stock. Indoor bioassay results showed that the highest mortality of M. incongnita was 89.7%,the log dosage probability line (LD-P) was as follows: y =-7.5989+1.717826 x,R^2=0.9960,the median lethal concentration (LC50) was 2.15×10^7 CFU/m L. The pot experiment results showed that the best control effect time was 14 days after drug treatment,with control efficiency of 15.38%-31.57%,there was dose-effect relationship,and it could promote the growth of cucumber rootstock. The greenhouse test results showed that,the best control effect time was 120 days after drug treatment,with control efficiency of 80.47%-84.38%. It was concluded that Acremonium sp. powder had control efficiency against M. incongnita,while it could promote the growth of cucumber rootstock. The powder must be applied in early when disease was lighter,and be applied again after 4 months.
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