猪繁殖与呼吸系统综合征病毒华南株GDgz的分离鉴定及遗传进化分析  

Isolation,identification and genetic variation analysis of South China strain GDgz of porcine reproductive and respiratory syndrome virus

作　　者：于林洋[1] 董建国[1,2] 张乐宜[1] 刘燕玲[1] 梁鹏帅[1] 王磊[1] 宋长绪[1] YU Lin-yang DONG Jian-guo ZHANG Le-yi LIU Yan-ling LIANG Peng-shuai WANG Lei SONG Chang-xu(College of Animal Science, South China Agricultural University, Guangzhou 510642, China College of Animal Husbandry and Veterinary, Xinyang College of Agriculture and Forestry, Xinyang 464000, China)

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]信阳农林学院牧医工程学院,河南信阳464000

出　　处：《广东农业科学》2017年第4期138-145,F0003,共9页Guangdong Agricultural Sciences

基　　金：国家科技支撑计划项目(2015BAD12B02-5)

摘　　要：为了解华南地区猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异特性,用Marc-145细胞从广东省疑似猪繁殖与呼吸综合征发病猪场采集的肺组织中分离得到1株PRRSV(命名为GDgz),该毒株能产生明显的细胞病变。全基因组进化分析和同源性比对分析结果显示,GDgz与中国高致病性毒株位于同一分支,与欧洲型毒株Lelystad-virus同源性最低、仅为60.3%,与美洲型经典毒株VR-2332的同源性为88.8%,与中国高致病性毒株JXA1和HuN4同源性为98.8% 和98.9%,与JXA1的疫苗株同源性为91.1%,与NADC30和NADC30-like毒株 CHsx1401和JL580同源性分别为84.5%、87.2%和83.6%。NSP2序列比对结果显示,GDgz在高变区存在30个不连续氨基酸缺失。GP5序列比对结果显示,GDgz在GP5抗原表位上有氨基酸突变,表明GDgz属于美洲型高致病性毒株传代致弱的疫苗株,且在抗原表位上有一定程度的突变。To understand the evolution of porcine reproductive and respiratory syndrome virus（PRRSV）in South China,we isolated a PRRSV strain in Marc-145 cell from lung of suspected PRRSV-positive pig in South China and named GDgz. GDgz strain could cause typical cenotaphic effect. The results of phylogenetic tree and homology alignment analysis showed that GDgz belonged to the same cluster with high pathogenetic PRRSV（HPPRRSV） strains in China;GDgz had a far distance with European strain Lelystad virus,the nucleotide homology was only 60.3%,with American classic strain VR-2332 was 88.8%,with Chinese HP-PRRSV strains JXA1 and Hu N4 were 98.8% and 98.9%,with the vaccine strain JXA1-P80 of JXA1 was 99.1%,with NADC30 and NADC30-like strains CHsx1401 and JL580 were 84.5%,87.2 and 83.6%,respectively. The result of sequence alignment showed that there were 30 discontinuous amino acids deletion in the high variable region of NSP2. These results showed that GDgz belonged to vaccine strain of high pathogenetic North American type PRRSV and had some mutations in the epitope region.

关 键 词：猪 繁殖与呼吸综合征病毒 分离鉴定 遗传进化 

分 类 号：S855.3[农业科学—临床兽医学]