FABP4在妊娠期高血压疾病胎盘滋养细胞免疫炎症反应中的作用研究  

Effect of FABP4 on placental trophoblastic immune inflammatory response in patients of hypertensive disorder complicating pregnancy

作　　者：毛彩艳[1] 张辉[2] 李旭生[3] 杨松昊 邓梅[1] 丁宁[1] 吴凯[1] 杨晓玲[1] 张慧萍[3] 姜怡邓[1] 

机构地区：[1]宁夏医科大学基础医学院,宁夏银川750004 [2]宁夏医科大学临床医学院,宁夏银川750004 [3]宁夏医科大学总医院,宁夏银川750004

出　　处：《中国药理学通报》2017年第8期1126-1131,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81570452;81660258);宁夏高等学校科学研究项目(No NGY2016129;NGY2016086)

摘　　要：目的探讨脂肪酸结合蛋白4(fatty acid binding protein 4,FABP4)在妊娠期高血压疾病胎盘滋养细胞免疫炎症反应中的作用。方法体外培养人胎盘滋养细胞株(HTR-8),分别加入0、10、100、500、1 000μmol·L^(-1)N-硝基-L-精氨酸甲酯(L-NAME),孵育48 h后,采用ELISA检测炎症因子IL-6、TNF-α改变;qRT-PCR、Western blot检测FABP4 mRNA和蛋白表达;构建FABP4腺病毒过表达载体转染细胞后,采用100μmol·L^(-1)L-NAME干预,ELISA分析炎症因子IL-6、TNF-α的变化。结果与对照组比较,L-NAME干预组IL-6、TNF-α及FABP4表达量明显增高(P<0.01);过表达FABP4组与对照组相比,炎症因子IL-6、TNF-α明显增加(P<0.01)。结论 FABP4参与了妊娠期高血压疾病胎盘滋养细胞免疫炎症反应的调控。Aim To investigate the role of fatty acid binding protein 4（ FABP4） in placental trophoblastic immune response in patients with hypertensive disorder complicating pregnancy. Methods The human placental trophoblast cell line（ HTR-8） was cultured in vitro and incubated with L-NAME for 0,10,100,500 and 1 000 μmol·L-1for 48 h. The levels of IL-6 andTNF-α were analysed by ELISA. The mRNA and protein expression levels of FABP4 were detected by Western blot and qRT-PCR. The cells transfected FABP4 adenovirus expression vector were intervented with 100μmol· L-1L-NAME. Then,the levels of IL-6 and TNF-α were determined by ELISA. Results Compared with the control group,the expression levels ofIL-6,TNF-α and FABP4 were significantly increased in the intervention group（ P〈0. 01）. Meanwhile,the expression levels of IL-6 and TNF-α were significantly increased in the overexpressed FABP4 group,compared with the control group（ P〈0. 01）. Conclusion FABP4 is involved in regulating the trophoblastic im-mune-inflammatory response in patients of hypertensive disorder complicating pregnancy.

关 键 词：脂肪酸结合蛋白4 妊娠期高血压疾病 滋养细胞 IL-6 TNF-α N-硝基-L-精氨酸甲酯 

分 类 号：R321.4[医药卫生—人体解剖和组织胚胎学] R392.12[医药卫生—基础医学]