聚合物纳米微球分离纯化米卡芬净母核的方法研究  

Studies on the Separation and Purification of Micafungin Nucleus by Nanoscale Polymer Particles

作　　者：邓佩佩[1] 谢新宇[1] 王晶珂[1] 郑学丽[1] 李梅[1] 李敏[1] 王小连[1] 

机构地区：[1]微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,石家庄050015

出　　处：《国外医药（抗生素分册）》2017年第4期190-192,共3页World Notes on Antibiotics

基　　金：基金项目:微生物来源药物发酵及分离纯化中试条件建设和质量管理体系(169676404G)

摘　　要：目的研究聚合物纳米微球分离纯化米卡芬净母核的方法。方法采用中压层析系统,以聚合物纳米微球为层析介质,以高纯度的米卡芬净母核收率为指标,筛选聚合物纳米微球的类型,优化洗脱条件。结果 Uni PS?30型聚合物纳米微球为最佳层析介质,洗脱剂为5%的乙醇溶液,流速为10m L/min、上样量1.0g/(100g介质)时,得到纯度>98%的米卡芬净母核精粉,总收率>85%。结论此分离纯化米卡芬净母核的方法简便、可靠,适用于工业化生产。Objective Study the methods of polymer nanoparticle separation and purification micafungin nucleus. Methods Using medium pressure chromatography system, the polymer nanospheres were used as chromatography medium and the yield of high purity micafungin nucleus as indexes. The type of polymer nanoparticle were selected and the elution conditions were optimized. Results The UniPS 30 type of polymer nanoparticles was selected as the best chromatography medium, and 5% ethanol solution was used as the elution solution at a flow rate of 10mL/min, and the sample loading amount was 1.0g/（100g medium）. The purity of micafungin nucleus was more than 98% and the yield was over 85%. Conclusion The separation and purification method is simple and reliable. Thus the process is suitable for industrial production.

关 键 词：聚合物纳米微球 米卡芬净母核 分离纯化 

分 类 号：Q936[生物学—微生物学]