化学发光免疫法与免疫放射法检测血清促甲状腺素的临床价值对比  

Clinical Value Comparison of Chemiluminescence Immunoassay and Immuno-radiometric Assay in Detection of Serum Thyrotropin

作　　者：孙亚东 

机构地区：[1]湖北省武汉市新洲区中医院检验科,湖北武汉430400

出　　处：《世界最新医学信息文摘》2017年第15期3-4,共2页World Latest Medicine Information Electronic Version

摘　　要：目的分析比较化学发光免疫法和免疫放射法两种方法在检测血清促甲状腺素中的价值。方法选取我院2015年6月至2016年6月登记救治的10例甲状腺疾病患者,取这10例患者的血清,分别用化学发光免疫法和免疫放射法检测10例患者的血清促甲状腺素水平。经过对两种方法检测结果的线性范围、批内、批间差异分析对比,评价哪一种方法检测结果更好价值性更高;分别用质控血清加入高、中、低的已知浓度中,用同样方法测定回收率以评价两种方法的回收率范围;采用相关系数分析化学发光免疫法和免疫放射法的相关性。结果化学发光免疫法检测的血清促甲状腺素线性范围0.9995比免疫放射法检测的线性范围0.9993更宽;化学发光免疫法检测的血清促甲状腺素批内变异系数2.2%比免疫放射法检测的批内变异系数3.2%更小;化学发光免疫法检测的血清促甲状腺素批间变异比免疫放射法检测的批间变异更小,化学发光免疫法重复性更好。两种方法的回收率范围92.2%~103.1%均达到临床要求,两种方法测定结果高度正相关。结论化学发光免疫法比免疫放射法在检测血清甲状腺素中更灵敏、准确、精确,为了判断疾病的准确率,化学发光免疫法应在各种疾病的临床检测中推广使用。Objective to analyze and compare value of chemiluminescence immunoassay and immunoradiometric assay in detection of serum thyrotropin. Methods choose 10 cases thyroid disease patients registered in our hospital from June 2015 to June 2016, detect their serum thyrotropin level with chemiluminescence immunoassay and immunoradiometric assay respectively Compare and analyze linear range, difference inside and between batches of detection results two methods, evaluate which method has better detection results and higher value. Add quality-controlled serum into high, medium and low known concentration, detect recovery rate by same method to evaluate range of recovery rate of two methods; analyze correlation between chemiluminescence immunoassay and immunoradiometric assay with related coefficient. Result linear range of serum thyrotropin detected by chemiluminescence immunoassay was 0.9995, wider than 0.9993 by immunoradiometric assay; variation coefficient inside batch of serum thyrotropin detected by chemiluminescence immunoassay was 2.2%, less than 3.2% by immunoradiometric assay; variation coefficient between batches of serum thyrotropin detected by chemiluminescence immunoassay was less than that by immunoradiometric assay, and chemiluminescence immunoassay has better repeatability. Recovery rate range of two methods was 92.2% to 103.1%, meeting clinical requirement. Results detected by two methods were highly positive correlated. Conclusion chemilmninescence immunoassay was more sensitive, accurate, precise detecting serum thyroxine than immunoradiomelric assay, which should be promoted and applied in clinical detection of various diseases to improve diagnosis rate.

关 键 词：化学放光免疫法 免疫放射法 血清 甲状腺素 对比 

分 类 号：R4[医药卫生—临床医学]