Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats  

作　　者：Guang-Yue Hou Li-Hui Men Lu Wang Zhong Zheng Zhi-Qiang Liu Feng-Rui Song Zhong-Ying Liu 

机构地区：[1]National Center for Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences [2]School of Pharmaceutical Sciences,Jilin University

出　　处：《Chinese Chemical Letters》2017年第6期1214-1219,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81373952,81473537);the Jilin province science and technology development projects(No.20150311039YY)

摘　　要：The effect of Radix Scutellariae treated on type 2 diabetic rats has been investigated by a liquid chromatography coupled with tandem mass spectrometry（LC-MS/MS） based urinary quantitative approach.In this research,multiple reactions monitoring mode of MS/MS in LC-MS/MS analysis was used to quantitatively analyze the concentrations of 7 endogenous compounds in urine of normal control group,type 2 diabetic model group and Radix Scutellariae-treated group,and multivariate statistical analysis was utilized for MS data processing.The above-mentioned three groups can be distinguished via pattern recognition.The obtained results indicated that Radix Scutellariae affect the urinary metabolic profiling of type 2 diabetic rats on the polyol pathway,protein glycation reaction and amino acids metabolism pathway.According to these results,Radix Scutellariae should have the pharmacological effect on preventing or delaying the onset and progression of diabetes and its complications.The effect of Radix Scutellariae treated on type 2 diabetic rats has been investigated by a liquid chromatography coupled with tandem mass spectrometry（LC-MS/MS） based urinary quantitative approach.In this research,multiple reactions monitoring mode of MS/MS in LC-MS/MS analysis was used to quantitatively analyze the concentrations of 7 endogenous compounds in urine of normal control group,type 2 diabetic model group and Radix Scutellariae-treated group,and multivariate statistical analysis was utilized for MS data processing.The above-mentioned three groups can be distinguished via pattern recognition.The obtained results indicated that Radix Scutellariae affect the urinary metabolic profiling of type 2 diabetic rats on the polyol pathway,protein glycation reaction and amino acids metabolism pathway.According to these results,Radix Scutellariae should have the pharmacological effect on preventing or delaying the onset and progression of diabetes and its complications.

关 键 词：LC-MS/MS Endogenous markers Multivariate statistical analysis Type 2 diabetic complications Radix Scutellariae 

分 类 号：R285.5[医药卫生—中药学]