α-硫辛酸对大鼠全横断脊髓损伤后GAP-43和Caspase-3表达的影响  

Effects of α-lipoic acid on the expression of GAP-43 and Caspase-3 after spinal cord injury in rats

作　　者：周治来[1] 俞洁[2] 黄子祥[3] 黄帅[3] 姚舜[3] 张辉[1] 

机构地区：[1]广东省第二人民医院脊柱外科,广州510317 [2]新疆军区总医院信息科,乌鲁木齐830000 [3]南方医科大学珠江医院骨科,广州510282

出　　处：《神经解剖学杂志》2017年第4期397-402,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金-青年科学基金(81400996);广东省自然科学基金-博士启动项目(2014A030310100)

摘　　要：目的:研究α-硫辛酸对脊髓全横断损伤(SCI)大鼠损伤部位神经生长相关蛋白(GAP-43)、神经细胞凋亡相关蛋白(Caspase-3)的表达,探讨α-硫辛酸对大鼠脊髓全横断损伤功能恢复的作用。方法:制作并评价SD大鼠SCI模型后,64只大鼠随机分为假手术组、脊髓损伤组(SCI组)、SCI+游泳训练组(游泳组)和SCI+α-硫辛酸组(硫辛酸组)。各组按手术后7、14、21、28 d等4个时间点收集标本,每个时间点大鼠均为4只。各组大鼠进行BBB评分。各组4个时间点的GAP-43和Caspase-3表达用免疫组化测定,同时用Western Blot检测GAP-43表达。结果:游泳训练和用硫辛酸均能提高脊髓损伤大鼠的BBB评分(P<0.05)。与SCI组比较,游泳组和硫辛酸组GAP-43表达显著增加,Caspase-3表达则明显降低(P<0.05),且硫辛酸组比游泳组的GAP-43表达增加和Caspase-3表达降低更为明显(P<0.05)。结论:游泳训练和α-硫辛酸可以上调脊髓损伤大鼠GAP-43表达和抑制Caspase-3表达促进损伤神经修复,对改善大鼠运动功能有一定疗效。Objective： To study the expression of nerve growth associated protein （GAP-43） and neuronal apoptosis related protein （Caspase-3） in rats with spinal cord injury （SCI）, and investigate the effect ofα-lipoic acid on functional recovery of SCI rats. Methods： SCI model of SD rats was made and evaluated. 64 rats were randomly divided into sham operation group, spinal cord injury group （SCI group）, SCI ＋ swimming training group （swimming group）, SCI ＋ α-lipoic acid group （lipoic acid group）. The specimens were collected at four, 14, 21 and 28 days after operation, and the rats in each time point were only 4. BBB （ Basso Beattie Bresnahan） score was used in each group of rats. The expression of GAP-43 and Caspase-3 at four time points in each group was examined by immunohistochemistry, and the expression of GAP-43 was detected by Western Blot. Results： Swimming training and lipoic acid could improve the BBB score of spinal cord injury rats （P 〈 0.05 ）. Compared with SCI group, the expression of GAP-43 was significantly increased and the ex- pression of Caspase-3 was significantly lower （P 〈 0.05 ） in swimming group and lipoic acid group, and the expression of GAP-43 in lipoic acid group was higher than that in swimming group, and the expression of Caspase-3 was decreased sig-nificantly （ P 〈 0.05 ）. Conclusion： The results show that swimming training andα-lipoic acid can increase the expression of GAP-43 and inhibit the expression of Caspase-3 in spinal cord injury and promote the repair of injured nerves in rats, and it can improve the motor function of rats.

关 键 词：Α-硫辛酸 游泳训练 脊髓损伤 GAP-43 CASPASE-3 大鼠 

分 类 号：R651.2[医药卫生—外科学]