中国储粮害虫DNA条形码鉴定系统研究  

Development and primary application of the DNA barcode identification system of grain pests

作　　者：吴志刚[1] 李文欣[1] 赵紫华[1] 伍祎[2] 张涛[2] 曹阳[2] 李福君[2] 李志红[1] 

机构地区：[1]中国农业大学植物保护学院,北京100193 [2]国家粮食局科学研究院,北京100037

出　　处：《中国农业大学学报》2017年第5期82-89,共8页Journal of China Agricultural University

基　　金：国家国际科技合作专项项目(2013DFG32350)

摘　　要：为一步提升我国储粮害虫快速、准确鉴定能力,采用微软产品技术解决方案,应用SQL SERVER 2008数据库和C#编程语言,研制了中国储粮害虫DNA条形码鉴定系统(Grain Pests DNA Barcode Identification System,GPDBIS)。GPDBIS收集了我国将近40种储粮昆虫及仓储螨类的基础信息和mtDNACOⅠ条形码信息,提供了基于DNA条形码的相似度比对鉴定功能和系统发育树分析鉴定功能、基础信息查询及管理功能。GPDBIS初步应用效果显示,该系统界面友好、功能稳定,能够实现我国常见储粮害虫的快速、准确鉴定。For rapid and accurate identification of stored-product pests,Grain Pests DNA Barcode Identification System （GPDBIS） was established by using SOL SERVER 2009 and C #. The basic information and mtDNACO I barcode information of about 40 species of stored-product pests and mites were collected in the GPDBIS system. This system provided similarity comparison, phylogenetic tree, information searching and management. The preliminarily application showed that the GPDBIS system was interface friendly and function stably, which achieved rapid and correct identification of stored-product pests. GPDBIS developed in this study could further enhance the ability of rapid and correct identification of stored-product pests, improved the monitoring and management of stored-product pests. It could also facilitate the international trade of agricultural goods and grain safety of China.

关 键 词：专家系统 储粮害虫 DNA条形码 相似度比对 系统发育树 

分 类 号：S433.89[农业科学—农业昆虫与害虫防治]