一种抗旱凤仙在PEG-6000模拟干旱胁迫下的应答反应  

The Drought-Stress Response of a Drought Resistant Impatiens Processed in PEG-6000 Solution in a Simulation Test

作　　者：廖雪兰[1,2] 文国琴[2] 刘庆林[1] 李雪杨[1] 邬梦郗 张莉[2] 潘远智[1] 

机构地区：[1]四川农业大学风景园林学院,成都611130 [2]西华师范大学珍稀动植物研究所,南充637002

出　　处：《分子植物育种》2017年第6期2375-2380,共6页Molecular Plant Breeding

基　　金：四川省"十三五"育种攻关项目(2016yzgg)资助

摘　　要：凤仙花是一种常见的庭院观赏花卉,适应性强,栽培历史悠久,本试验中所用的材料是普通凤仙花经过航天诱变后获得的突变体后代的凤仙花,在生理性状上表现出了和普通凤仙的差异性,激发人们对差异形成原因的探索。本试验利用不同浓度PEG-6000溶液处理诱变凤仙和普通凤仙,测定模拟干旱下两种凤仙花的发芽率、逆境相关保护酶活性、干旱下游基因SOS2、RD29B表达量的差异性。试验发现,诱变凤仙花的发芽率与普通凤仙花没有显著差异性,但是相关保护酶活性、MDA、干旱下游基因SOS2、RD29b表达量均有明显差异,试验表明诱变凤仙比普通凤仙更具有优势,在园林绿化方面具有更高的应用价值。Impatiens is a commonly seen garden flower, renowned for its strong adaptability and long history of cultivation. In this experiment, the Impatiens is selected from those which experienced aerospace mutation. It has been found that it is superior to the non-mutagenized Impatiens in terms of drought resistance and demonstrates tremendous differences from the normal Impatiens in physiology, which intrigues scholars to search for the underlying reasons. By reference to Impatiens balsamina L., this experiment used mutagenized Impatiens seeds,processed by PEG-6000 in different solution concentration, to measure the germination rate of Impatiens, its relative enzymatic activity and expression differences between gene SOS2 and gene RD29 b in the drought lower reaches. Under simulated drought stress, there is no distinct difference between the mutagenized Impatiens and the normal Impatiens in terms of germination rate. But by measuring the relative enzymatic activity, MDA, and the expression differences between gene SOS2 and gene RD29 b in the drought lower reaches, it is verified that the mutagenized Impatiens has more advantages than the normal Impatiens, and it can further cultivate become drought resistance varieties Impatiens. The so obtained Impatiens has higher application value in garden virescence.

关 键 词：诱变凤仙 逆境 PEG-6000 基因表达 

分 类 号：S681.1[农业科学—观赏园艺]