温度及盐胁迫对地梢瓜种子萌发及抗氧化酶活性的影响  

Effect of Temperature and Salt Stress on Seed Germination and Antioxidant Enzyme Activities of Cynanchum thesioides (Freyn).K.Schum

作　　者：张晓艳[1] 杨忠仁[1] 郝丽珍[1] 张凤兰[1] 郑清岭 郭霏[1] 翟学婧 

机构地区：[1]内蒙古农业大学农学院内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室,呼和浩特010019

出　　处：《西北植物学报》2017年第6期1166-1174,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：内蒙古自然科学基金(2015MS0359);内蒙古农业大学博士启动基金(BJ09-17);内蒙古农业大学科技创新(培育)团队(NDPYTD2013-3);公益性行业(农业)科研专项(201203004);内蒙古主席基金

摘　　要：以地梢瓜种子为试料,研究不同温度(5~45℃)及不同浓度NaCl胁迫(0~300mmol·L^(-1))对种子萌发及其抗氧化酶活性的影响。结果表明:(1)随着温度升高,种子各萌发指标呈先升高后降低的趋势,过高或过低均显著抑制种子正常萌发,萌发最适温度为25℃,在5℃和35℃条件下种子恢复萌发率均达95%以上。(2)随着NaCl浓度升高,种子萌发率、萌发势、活力指数呈显著下降趋势,而其相对盐害率呈增加趋势,且在300mmol·L^(-1)NaCl时萌发受到完全抑制;种子根系活力仅在高浓度NaCl(>150mmol·L^(-1))下受到显著抑制;种子在解除盐胁迫后可一定程度恢复萌发,但最终依然受到盐胁迫的显著抑制,且盐浓度越高抑制越严重,其耐盐适宜浓度为65.25mmol·L^(-1)。(3)种子萌发率与盐胁迫下POD、APX活性呈显著正相关关系,而与相应O_2~生成速率、MDA含量呈显著负相关关系;种子活力指数与盐胁迫下CAT活性呈显著负相关关系。研究发现,地梢瓜种子萌发受到NaCl胁迫的显著抑制,保护酶POD与APX在盐胁迫种子萌发过程中发挥着主要抗氧化作用;地梢瓜适于在中度盐渍土中萌发生长。The effects of different temperature （5-45 ℃） and NaCl （0-300 mmol·L-1） on seed germination index and antioxidant enzyme activities of Cynanchum thesioides（Freyn）.K.Schum were studied.The results showed that： （1） with the increase of temperature, germination index of seed all increased first and then decreased.The low or high temperature inhibited significantly seed germination.The optimum temperature for germination was 25 ℃, and its recovery germination ratio was over 95% at 5 ℃ and 35 ℃.（2） As NaCl concentration went up, the germination ratio, germination potentiality, vigor index of seed decreased, but relative salt injury rate increased and germination was completely inhibited at 300 mmol·L-1.The root vigor of seed was inhibited significantly only at high NaCl concentration（〉150 mmol·L-1）, the seed could recover germination after the salt stress was relieved, but it was still inhibited by salt stress.The higher salt concentration, the more prohibitive, and the appropriate content of salt-tolerant was 65.25 mmol·L-1（the salt content is 0.38%）.（3） Under salt stress, the germination ratio was positively correlated with POD and APX, but negatively correlated with O-·2 generation rate and MDA, the vigor index was negatively correlated with CAT.Therefore, the seed germination was significantly inhibited by salt stress, protective enzymes POD and APX played a major antioxidant role during seed germination under salt stress, Cynanchum thesioides（Freyn）.K.Schum was suitable for germination in moderate saline soil.

关 键 词：地梢瓜 温度 NACL 种子萌发 抗氧化酶活性 

分 类 号：Q945.78[生物学—植物学]