活性氧和能量调控下草莓果实衰老与超微弱发光的关系  

Relationship between Senescence and Ultraweak Photon Emission under Controlling of Reactive Oxygen and Energy in Strawberry Fruit

作　　者：刘欢[1] 梁爽[1] 闫宇彤 白杨[1] 郭金丽[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《西北植物学报》2017年第6期1182-1188,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31260455)

摘　　要：该试验以‘红颜’草莓果实为试材,用过氧化氢(H_2O_2)和茶多酚(TP)、呼吸链解偶联剂——2,4-二硝基苯酚(DNP)和三磷酸腺苷(ATP)处理草莓果实,研究促进和清除活性氧以及抑制能量生成和促进能量条件下,草莓果实衰老进程和超微弱发光(UPE)的变化,探讨UPE与草莓果实衰老的关系,为了解UPE与植物成熟衰老的关系提供理论依据。结果表明:(1)在草莓果实采后衰老过程中,果实硬度持续下降,失重率和腐烂率持续上升,同时其UPE强度下降。(2)H_2O_2处理和DNP处理的果实硬度均低于对照,而其失重率和腐烂率均高于对照,同时两种处理果实的UPE强度均低于对照。(3)茶多酚处理和ATP处理的果实硬度均高于对照,而其失重率和腐烂率均低于对照,同时两种处理的UPE强度均高于对照。研究发现,活性氧调控和能量调控均能影响草莓果实的UPE强度和衰老程度,活性氧的增加加剧了果实衰老和UPE强度下降,而清除活性氧则延缓了果实衰老和UPE强度下降;抑制ATP生成加剧了果实衰老进程和UPE强度下降,增加ATP则延缓了果实衰老和UPE强度下降;UPE强度随着草莓果实逐渐衰老而下降,UPE强度与草莓果实衰老有关,反映了草莓果实的衰老进程。The study was to discuss the relationship between Ultraweak photon emission （UPE） and senescence of strawberry fruit to supply theoretical basis for understanding the relationship between plant senescence and UPE.The study layout the experiment by soaking respectively postharvest ＇Hongyan＇ strawberry fruit with hydrogen peroxide （H2O2） and tea polyphenol（TP）, uncoupler 2,4-dinitrophenol （DNP） and adenosine triphosphate（ATP）,then analyze contrastively the variation of fruit ageing process and UPE under promoting reactive oxygen and eliminating reactive oxygen conditions, inhibiting energy formation and increasing energy conditions in postharvest strawberry fruit.Therefore the study aim to understand the relationship between UPE and senescence in strawberry fruit.The result showed that： （1） during fruit ageing process, fruit firmness of CK, H2O2 and TP treatments, DNP and ATP treatments decrease continuously weight loss rate and decay rate of above treatments increase continuously.Meanwhile UPE intensity of five treatments decrease.Based on above results, in fruit ageing process, UPE intensity decline along with the ageing of postharvest fruit.（2） In whole fruit ageing process, the fruit firmness of H2O2 and DNP treatments are all lower than those of CK and weight loss rate and decay rate are all higher than those of CK, meanwhile UPE intensity of H2O2 and DNP treatments are all lower than those of CK.（3） The fruit firmness of TP and ATP treatments are all higher than those of CK and weight loss rate and decay rate are all lower than those of CK, meanwhile UPE intensity of TP and ATP treatments are all higher than those of CK.Above results indicated that regulating reactive oxygen level and energy level can effect on ageing process and UPE intensity of fruit.Promoting reactive oxygen accumulation lead to speed up the ageing extent and to intensify the decline of UPE intensity of fruit, and eliminating reactive oxygen lead to delay the ageing extent and to relieve the decline of

关 键 词：草莓果实 活性氧调控 能量调控 衰老 超微弱发光 

分 类 号：Q945.65[生物学—植物学] S668.4[农业科学—果树学]