抗草甘膦转基因大豆对雄性生殖损伤小鼠体外受精作用的影响  

Effects of glyphosate-resistant transgenic soybean on in vitro fertilization of male mice with reproductive damage

作　　者：芦春斌[1,2] 张雁[1] 陈博慧[1] 林泽斌[1] 仇平乐 刘标[2] 

机构地区：[1]暨南大学发育与再生生物学系,广东广州510632 [2]环境保护部南京环境科学研究所,江苏南京210042

出　　处：《浙江农业学报》2017年第6期910-916,共7页Acta Agriculturae Zhejiangensis

基　　金：转基因生物新品种培育重大专项(2016ZX08012005);环保公益性行业科研专项(201509044)

摘　　要：用化疗药物环磷酰胺(CP)处理小鼠建立雄性生殖损伤模型,以抗草甘膦转基因大豆饲料喂食30 d,进行体外受精实验,检测其受精率及2-细胞、4-细胞、8-细胞和囊胚的形成率和优胚率,评估转基因大豆饲料对雄鼠生殖功能的潜在影响。实验结果显示,在CP处理导致的生殖损伤条件下,与非转基因大豆喂食组相比,以抗草甘膦转基因饲料喂食30 d对小鼠受精率,及其分裂形成的2-细胞、4-细胞、8-细胞和囊胚形成率和优胚率无显著(P>0.05)影响,表明CP诱导的生殖损伤状态下,抗草甘膦转基因大豆饲料喂食30 d不会对雄性小鼠的生殖功能造成损伤。To assess the effects of glyphosate-resistant transgenic soybean on reproductive function in mice with reproductive damage male mice were injected chemotherapeutical agent,cyclophosphamide（ CP）,to induced reproductive damage as model mice. The formation rate of zygotes,2-cells,4-cells,8-cells as well as blastocysts and excellent embryo rate were evaluated in vitro fertilization after feeding with glyphosate-resistant soybean for 30 days.The results showed that no significant difference（ P〉0. 05） in the formation rate of zygotes,2-cells,4-cells,8-cells,as well as blastocysts and excellent embryo rate were observed in cyclophosphamide（ CP） treated reproductivedamaged mice fed with glyphosate-resistant soybean for 30 days,compared with the mice fed with traditional soybean. In conclusion,there was no adverse effect on the reproductive function in male mice after feeding with transgenic soybean for 30 days under CP induced pathologic conditions.

关 键 词：转基因大豆 小鼠 环磷酰胺(CP) 体外受精 

分 类 号：S816.42[农业科学—饲料科学] S561.1[农业科学—畜牧学]