毛细管电泳-质谱联用技术及其在药物和生物分析中的应用  

Recent Advances in Capillary Electrophoresis-Mass Spectrometry and Its Application in Pharmaceutical and Biological Analysis

作　　者：周韦[1] 刘易昆 陈子林[1] 

机构地区：[1]武汉大学药学院,湖北武汉430071

出　　处：《质谱学报》2017年第4期362-374,共13页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(81573384;21375101)资助

摘　　要：毛细管电泳-质谱(CE-MS)联用技术是在液相色谱-质谱联用技术基础上发展起来的一项新型分析技术,它结合了毛细管电泳具有的分离效率高、分离速度快、样品消耗量少以及质谱检测具有的高灵敏度和强结构解析能力等优点,现已成为倍受分析化学工作者关注的新型微量分析技术。目前,CE-MS联用技术是中药有效成分分析,体内药物分析以及生物样品,如氨基酸、多肽、蛋白质和多糖等分析的重要手段。本文对CE-MS联用技术中同轴鞘流及无鞘流纳流电喷雾等几种接口装置的研究进展,CE-MS技术在中药活性成分分析及多级质谱结构解析以及氨基酸、多肽及蛋白质等生物样品分析中的应用研究进行了综述,并对该技术的发展进行了展望。Capillary electrophoresis-mass spectrometry （CE-MS） , developed on the basis of liquid chromatography-mass spectrometry, is a new hyphenated technique that combines the advantages like high separation efficiency, short analytical time, low sample consumption in CE and the high sensitivity, powerful molecular structure eluci-dation in MS. It has been paid great attention by analytical scientists and become a pow-erful tool for analysis of active components in Chinese medicine, in vivo drugs and bio-samples like amino acids, peptides, proteins and polysaccharides. In this paper,a brief review was given on recent advance in CE-MS and its applications, including advance in sheath-flow and sheathless nano-spray interfaces and applications in analysis of pharma-ceutical and biological samples. The first part of this review summarizes the develop-ment of stable and efficient interfaces to improve the feasibility of CE-MS technique. Several interfaces like coaxial sheath-flow, electrokinetic sheath-flow, liquid injection and sheathless interface with etching emitter have been reviewed. The second part intro-duces the application of CE-MS in the past few years. The applications are categorized according to the types of analytes, including the analysis for active components in Chi-nese medicines, in vivo drugs, amino acids, peptides, proteins and carbohydrates. Coat-ings for capillary inner wall, online processing strategies, sample preparation methods and other experiment methods have been discussed in each category. In the last part of this review, a perspective of this technique has been discussed.

关 键 词：毛细管电泳-质谱(CE-MS) 电喷雾离子化接口装置 药物分析 生物分析 综述 

分 类 号：O657.63[理学—分析化学]