自制爬片装置用于细胞爬片及细胞染色  

New method of preparation cell-attached slides for staining with self-made device

作　　者：苏获[1] 毛林锋[2] Benson Kaluba 段智曦 刘擎[2] 黄鹏[2] 

机构地区：[1]中南大学护理学院,湖南长沙410013 [2]中南大学湘雅医院,湖南长沙410008 [3]中南大学湘雅二医院,湖南长沙410011

出　　处：《中国现代医学杂志》2017年第15期38-41,共4页China Journal of Modern Medicine

摘　　要：目的利用自制爬片装置改进细胞爬片的制备方法。方法通过自制装置和传统方法制备细胞爬片,苏木精-伊红染色法(HE染色)观察细胞生长状态,比较两种细胞爬片制备方法的差异;利用自制装置制备同时给予3种不同干预处理的细胞爬片,验证其应用于制备多干预条件细胞爬片;制备同时含有3种不同细胞的集成爬片,验证其用于制作细胞集成爬片的应用。结果自制装置可简化细胞爬片流程,细胞直接贴于载玻片上生长,未出现污染和增殖受限,制作多干预或多种细胞集成爬片效果明确,无相互干扰现象。结论自制装置能更简便地制备以满足普通染色及特殊染色实验要求的细胞爬片,是一种更好的制备细胞爬片的方法。Objective To provide an improved method to prepare cell-attached slides. Methods Cell-attached slides were prepared by routine method and self-made device, and the difference between the two methods were compared by observing the cell growth after HE staining. Three kinds of interventions were given to the same cell-attached slide to test the application in making different interventions cell-attached slide of the device. Three kinds of cells were planted in the same slide to test the application in making integrated cell-attached slides of the device. Results Our device simplified the preparation of cell-attached slides. Cells grown on the glass and never showed pollution or growth restriction, given different interventions or planted different cells at the same time without interaction. Conclusions The new method simplifies the processes of preparation cell-attached slides, and the slides made by our device satisfies the experiment demands of all kinds of cells staining, makes it as a more effective method than before.

关 键 词：细胞爬片 细胞培养 染色 

分 类 号：Q2-33[生物学—细胞生物学]