拟南芥黄化突变体k60的基因作图定位  

Genetic mapping of a chlorosis mutant k60 in Arabidopsis thaliana

作　　者：戴楠[1] 袁光孝[1] 刘小敏[1] 高宏波[1] DAI Nan YUAN Guang-xiao LIU Xiao-min GAO Hong-bo(College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P. R. China.)

机构地区：[1]北京林业大学生物科学与技术学院

出　　处：《北京林业大学学报》2017年第7期40-45,共6页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31501090;J1103516)

摘　　要：拟南芥黄化突变体k60是从甲基磺酸乙脂诱变的拟南芥突变体库中筛选得到的。该突变体表现为叶色发黄,生长迟缓,叶绿素含量降低。遗传分析发现其为单基因控制的隐性突变。利用遗传作图的方法将突变基因定位于拟南芥5号染色体上CH5-6.0到CH5-6.24两个分子标记之间的231 kb区间内。本项工作的研究结果为该突变基因的鉴定奠定了前期基础,并且为研究拟南芥黄化基因的功能提供了新的实验材料。An Arabidopsis thaliana mutant k60 with a chlorosis symptom was obtained by screening an ethyl methane sulphonate （EMS） mutagenized mutant population. This mutant displayed a phenotype of yellow leaf, slow growth and reduction of chlorophyll content. Genetic analysis indicated that the mutant phenotype was recessive and controlled by a single gene. k60 was mapped to a region of 231 kb between molecular markers CH5-6. 043 and CH5-6. 127 on chromosome 5 by genetic mapping. The results in this study lay the basis for the identification of the mutant gene, and provide a new material for the study of the function of chlorosis genes in Arabidopsis.

关 键 词：拟南芥 黄化突变体 k60 基因作图定位 

分 类 号：Q943.2[生物学—植物学]