补骨脂酚对UVB诱导HaCaT细胞凋亡因子p53和caspase-3表达的影响  

Effect of Bacuchiol on UVB Induced HaCaT Cell Apoptosis and p53,Caspase-3 Expression

作　　者：孙琪[1] 朴成玉[2] 陈丹丹[3] 石晨曦[1] 刘永武[2] 穆阳[4] 曹玲[5] 张宁[2] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040 [3]黑龙江中医药大学第二临床医学院,黑龙江哈尔滨150040 [4]佳木斯大学人文学院,黑龙江佳木斯154007 [5]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国美容医学》2017年第7期37-40,共4页Chinese Journal of Aesthetic Medicine

基　　金：教育部"春晖计划"合作科研项目(Z2010018)

摘　　要：目的:探讨补骨脂酚对UVB诱导HaCaT细胞凋亡因子p53和caspase-3表达的影响及其机制。方法:四甲基噻唑蓝[3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl Tetrazolium Bromide,MTT]法检测不同浓度补骨脂酚对正常细胞增殖的影响。实验设计分为四组,空白组、模型组、雌二醇组及补骨脂酚组。以照射强度0.61m W/cm^2,照射时间10min,建立HaCaT细胞凋亡模型;MTT法检测各组细胞增值率;活性氧(Reactive Oxygen Species,ROS)试剂盒检测各组细胞中ROS含量变化;线粒体膜电位检测试剂盒(JC-1)检测各组细胞线粒体膜电位变化;实时定量PCR(Reverse transcriptional PCR,RT-PCR)检测各组细胞中caspase-3 mRNA表达水平;蛋白免疫印迹法(Western Blot)检测各组细胞中p53、caspase-3蛋白表达水平。结果:10-6mol/L补骨脂酚组细胞增值率无明显变化(P>0.05),但是可降低ROS含量、线粒体膜电位、caspase-3mRNA表达量、p53及caspase-3蛋白表达量(P<0.05)。结论:补骨脂酚可抑制UVB诱导的HaCaT细胞凋亡,其作用机制主要是通过降低细胞内活性氧的含量、降低细胞线粒体膜电位、抑制p53及caspase-3的表达。Objective To study the effect of bacuchiol on UVB induced HaCaT cell apoptosis factors of p53, caspase-3 expression and its influence mechanism. Methods The experimental design was divided into four groups： blank group, model group, estradiol group and psoralen phenol group. HaCaT cell apoptosis model was established by irradiation intensity of 0.61 mW/cm^2 and irradiation time of 10 min, determined by MTT method to detect the activity of each cell, mitochondrial membrane potential detection kit （JC-1） each cell mitochondrial membrane potential change, in each cell of caspase-3 mRNA expression were detected by RT-PCR, in each cell of p53 and caspase-3 protein expression were detected by Western Blot. Results The best effective bacuchiol concentration on HaCaT cell no promotion and inhibition（P〉0.05）, but it plays a protective role of HaCaT cells apoptosis model, and reduces the UVB induced in the process of ROS contend and mitochondrial membrane potential and p53, caspase-3 mRNA and protein expression（P〈0.05）. Conclusion Bacuchiol suppress the human skin HaCaT cells apoptosis induced by UVB, its mechanism is mainly inhibiting the p53 and caspase-3 of expression.

关 键 词：补骨脂酚 UVB HACAT 凋亡 P53 caspase-3 

分 类 号：R285.5[医药卫生—中药学]