脂氧素A4对睾丸扭转大鼠CD4^+ T细胞的调节和保护作用  

Effect of Lipoxin A4 on CD4^+ T Cell in Rat Testicular Torsion

作　　者：陈元[1,2] 朱登安[1,2] 杨奇盛[1] 周贤龙[1] 赵剡[1] 宋小兵[1] 沈俊[1] 张志远[1] 刘靖[1] 涂小鹏 

机构地区：[1]武汉大学中南医院急救中心,湖北武汉430071 [2]武警湖北总队医院急诊科,湖北武汉430061

出　　处：《武汉大学学报（医学版）》2017年第5期732-734,749,共4页Medical Journal of Wuhan University

基　　金：湖北省卫生和计划生育委员会一般项目(编号:WJ2015MB106)

摘　　要：目的:研究脂氧素A4在睾丸缺血-再灌注损伤过程中对免疫细胞CD4^+ T的调节,保护生殖细胞功能作用。方法:健康成年大鼠40只随机分成A组(假手术组)、B组(睾丸扭转组)、C组(扭转复位组)、D组(扭转复位脂氧素A4治疗组),每组各10只。于术后24h分别检测患侧睾丸组织中IFN-γ、IL-2、IL-4、IL-10的浓度,以及睾丸组织中MDA、SOD、GPx含量。对各组睾丸组织行生殖细胞凋亡检测,行HE染色和Johnsen功能评分等形态学观察。结果:与A组相比,睾丸扭转后细胞因子IFN-γ、IL-2和组织中MDA显著升高(P<0.05),而SOD、GPx明显降低。与B和C组比较,D组细胞因子IL-4、IL-10,以及组织中SOD、GPx显著升高(P<0.05),细胞因子IFN-γ、IL-2和睾丸组织MDA水平降低明显。与B和C组比较,D组睾丸凋亡指数明显降低(P<0.05),Johnsen评分明显升高(P<0.05)。透视电镜下,B组和C组生殖细胞内线粒体内嵴结构破坏,溶酶体肿胀,细胞间隙增宽,D组细胞结构完整。结论:在睾丸缺血-再灌注过程中LXA4可以通过调节CD4^+ T细胞炎性因子的释放,减轻再灌注损伤炎症对生殖细胞的损伤,对生殖功能起保护作用。Objective：To study the immune regulation effect of Lipoxin A4（LXA4）on CD4＋ T cells and the protective effect on germ cells in testicular ischemia and reperfusion injury in rats.Methods：Forty rats were randomly divided into 4groups：sham group（A）;testicular torsion group（B）;torsion/detorsion（T/D）group（C）,T/D plus LXA4-pretreated group（D）.Biochemical analysis（IFN-γ,IL-2,IL-4,IL-10,MDA,SOD and GPx in the testes）,apoptosis assessment of germ cells,and morphologic evaluation were carried out after orchiectomies.Results：Compared with group A,the levels of proinflammatory cytokines（IFN-γ,IL-2）and MDA were significantly increased in the testes（P〈0.05）,and the levels of SOD and GPx were decreased after testicular torsion.Compared with B and C,the levels of IL-4,IL-10 and tissue SOD/GPxwere significantly increased（P〈0.05）in group D,while IFN-γ,IL-2and MDA levels were significantly decreased.In addition,the AI（apoptosis index）values of group D was significantly lower than group B and C（P〈0.05）,whereas Johnsen score was significantly increased（P〈0.05）.Furthermore,swollen mitochondria with degenerated cristae and enlarged intercellular spaces were observed under transmission electron microscopy in group D.Conclusion：LXA4can regulate cytokine secretion of CD4~＋ T cells and reduce the inflammation injure to germ cells,thus protecting reproductive function in testicular ischemia-reperfusion in rats.

关 键 词：脂氧素A4 CD4+ T细胞 睾丸扭转 保护作用 

分 类 号：R697[医药卫生—泌尿科学]