MUC5AC和TNF-α在大鼠急性分泌性中耳炎模型中的表达变化  

Expression of MUC5AC and TNF-α in Middle Ear Mucosa in a Rat Model of Acute Otitis Media With Effusion

作　　者：王梦航[1] 李水静[1] 韩琳[2] 余力生[2] WANG Menghang Lee Su Jeong Han Lin YU Lisheng(Department of Otolaryngology Head and Neck Surgery, Peking University International Hospital, Peking University, Beijing, 102206, China Department of Otolaryngology Head and Neck Surgery, People＇ s Hospital, Peking University, Beijing 100044, China)

机构地区：[1]北京大学国际医院耳鼻咽喉-头颈外科,北京102206 [2]北京大学人民医院耳鼻咽喉-头颈外科,北京100044

出　　处：《中华耳科学杂志》2017年第3期329-333,共5页Chinese Journal of Otology

基　　金：十二五国家科技支撑计划(2012BAI12B01);国家自然科学基金科学基金项目(11202018);中国博士后科学基金项目(20110490269;2013T60055);北京大学国际医院院内科研基金(YN2016QN03)~~

摘　　要：目的研究MUC5AC(黏蛋白MUC5AC)和TNF-α(肿瘤坏死因子-α)在大鼠中耳炎黏膜的表达变化。方法40只SD大鼠随机分为对照组10只、实验组30只;实验组建立大鼠急性分泌性中耳炎模型,采用q PCR检测MUC5AC及TNF-α在第1天,第3天,第7天两组中耳黏膜的表达变化,并用免疫组织化学染色技术分别检测MUC5AC和TNF-α在第1天,第3天,第7天在中耳黏膜的表达变化。结果在对照组中,MUC5AC不表达,TNF-α轻度表达,实验组从第1天到第3天MUC5AC及TNF-α的表达随着时间的延长而增强,至第7天则表达开始减弱,实验组与对照组的比较差别具有统计学意义(P<0.05)。结论 MUC5AC和TNF-α在大鼠急性分泌性中耳炎黏膜中表达上调,且与时间相关。Objective To study expression of MUC5 AC and TNF-α in the middle ear mucosa in rat model of acute otitis media with effusion.Methods Forty adult female Sprague-Dawlay（SD） rats were randomly divided into a control group and a experimental group.Rats in the experimental group received injection of endotoxin.The level and pattern of MUC5 AC and TNF-α expression within the middle ear epithelium were tested by q PCR and immunohistochemical assays.Results q PCR and immunohistochemical staining results showed increased expression of MUC5 AC and TNF-α in the experimental group along with time（＊P〈0.05） and the MUC5 AC were mainly concentrated on the goblet cells.Conclusions Expression of MUC5 AC and TNF-α is increased in rat middle ear epithelium with acute media otitis with effusion.Hence,it is speculated that MUC5 AC and TNF-α may participate in the process of acute media otitis with effusion.

关 键 词：MUC5AC TNF-Α 急性分泌性中耳炎 中耳黏膜 

分 类 号：R764[医药卫生—耳鼻咽喉科]