P-EGFR、P-ERK和NF-κB在中耳胆脂瘤上皮中的表达和意义  

Expression and Significance of P-EGFR,P-ERK and NF-κB in Middle Ear Cholesteatoma Epithelium

作　　者：王晓丽[1] 谢淑敏[2] 王月辉[3] 刘伟[4] 殷团芳[4] 彭安全[4] 任基浩[4] WANG Xiali XIE Shumin WANG Yuehui LIU Wei YIN Tuanfang PENG Anquan REN Jihao(Hunan Provincial Maternal and Child Health Care Hospital, Department of Pediatric Ophthalmology and Otorhinolaryngology, Changsha, Hunan Province, China （410008 Department of Otolaryngology-Head and Neck Surgery, The Xiangya Hospital, Central South University, Changsha, Hunan Province, China （410008 Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province, China （471000 Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Otology Research Institute of Central South University, Changsha, Hunan Province, China410011）.)

机构地区：[1]湖南省妇幼保健院儿童五官科,长沙410008 [2]中南大学湘雅医院耳鼻咽喉头颈外科,长沙410008 [3]河南科技大学第一附属医院耳鼻咽喉头颈外科,洛阳471000 [4]中南大学湘雅二医院耳鼻咽喉头颈外科中南大学耳科研究所,长沙410011

出　　处：《中华耳科学杂志》2017年第3期339-344,共6页Chinese Journal of Otology

基　　金：国家自然科学基金(项目批准号:81400457)~~

摘　　要：目的研究磷酸化表皮生长因子受体(P-EGFR)、磷酸化细胞外信号调节激酶(P-ERK)和细胞核转录因子κB(NF-κB)在中耳胆脂瘤上皮组织中的表达情况,探讨EGFR/ERK/NF-κB信号通路在中耳胆脂瘤发病机制中的作用。方法应用免疫组织化学SP法及Western blot(WB)免疫印迹法检测30例中耳胆脂瘤标本及15例正常外耳道皮肤组织中P-EGFR、P-ERK和NF-κB蛋白的表达。结果(1)P-EGFR蛋白阳性表达主要定位于细胞质和(或)细胞膜,在胆脂瘤组的阳性表达率为63%,明显高于正常外耳道皮肤组的20%(P<0.05);P-ERK蛋白阳性表达主要定位于细胞质和(或)细胞核,在胆脂瘤组的阳性表达率为70%,明显高于正常外耳道皮肤组的20%(P<0.05);NF-κB蛋白阳性表达主要定位于细胞核,在胆脂瘤组的阳性表达率为60%,明显高于正常耳道皮肤组的13.3%(P<0.05)。在30例中耳胆脂瘤组织中,P-EGFR、P-ERK和NF-κB两两之间呈正相关关系(P<0.05)。(2)Western blot免疫印迹法检测结果显示:中耳胆脂瘤组中P-EGFR、P-ERK和NF-κB的表达量明显高于正常外耳道皮肤组的表达量。结论 P-EGFR、P-ERK和NF-κB在中耳胆脂瘤上皮中表达增强,EGFR/ERK/NF-κB信号通路可能在中耳胆脂瘤组织的形成和发展过程中起重要作用。Objective To study the expression of P-EGFR,P-ERK and NF-κB in middle ear cholesteatoma epithelium,and explore the potential roles of EGFR/ERK/NF-κB signaling transduction pathway in middle ear cholesteatoma epithelial hyperplasia.Methods SP immunohistochemical assay and Western blot were used to detect the expression and significance of P-EGFR,P-ERK and NF-κB in 30 cases of middle ear cholesteatoma tissue samples and 15 cases of normal ear skin specimens.Results（1） Immunohistochemistry showed P-EGFR was expressed in the cytoplasm and/or membrane of epithelial cells.P-EGFR was positive in 19 of the 30 middle ear cholesteatoma specimens（63%）,compared to 3 of the 15 specimens（20%） in the control group（P〈0.05）.P-ERK expression in cholesteatoma was detected at a higher rate（21/30,70%） than normal external ear canal skin（3/15,20%）（P0.05）.NF-κB immunoreactivity was detected in the nuclei of epithelial cells at a higher rate in cholesteatoma（18/30,60%） than the control group（2/15,13.3%）（P〈0.05）.In middle ear cholesteatoma epithelium,there was a positive correlation between expression of P-EGFR,P-ERK and NF-κB（P〈0.05）.（2） Western blot discovered that the expression of P-EGFR,P-ERK and NF-κB in cholesteatoma was significantly more than the amount of expression in skin.Conclusions The expression of P-EGFR,P-ERK and NF-κB in middle ear cholesteatoma was significantly higher than normal skin.Activation of EGFR/ERK/NF-κB signaling transduction pathway may be involved in the information and development of middle ear cholesteatoma.

关 键 词：中耳 胆脂瘤 P-EGFR P-ERK NF-ΚB EGFR/ERK/NF-κB信号通路 

分 类 号：R764[医药卫生—耳鼻咽喉科]