不同因素对白檀试管苗增殖的影响  

Influence of the Different Factors on Proliferation Culture of Plantlets in Vitro of Symplocos paniculata

作　　者：安佰义[1] 包文慧 赵赫[1] 汤昊[1] 吴双[1] 韩春钢 陈丽[1] 宁崴[1] 殷佳慧[1] 王嘉莉 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]吉林市松花湖风景区园林管理处,吉林吉林132018 [3]长春市朝阳区园林管理处,吉林长春130021

出　　处：《园艺与种苗》2017年第4期6-9,共4页Horticulture & Seed

基　　金：吉林省科技发展计划重点科技攻关项目(20150204045NY)

摘　　要：[目的]筛选出白檀试管苗增殖的最适影响因子。[方法]以白檀(Symplocos paniculata)试管苗为试验材料,研究了玉米素质量浓度、基本培养基、接种方式及培养温度对白檀试管苗增殖的影响。[结果]以WPM为基本培养基,附加1.7 mg/LZT可使平均增殖系数达5.90;用双芽茎段做培养材料,平均增殖系数可提高至6.63;培养温度25/15℃(昼/夜)更利于试管苗增殖,10℃温差可使苗生长健壮,平均增殖系数达6.99。[结论]研究结果为白檀工厂化育苗提供了理论依据。[Objective] In order to screen out the optimal influence factors of proliferation for Symplocos paniculata plantlets in vitro. [Method] The influence of different ZT concentrations, basic mediums, inoculation methods and culture temperature on proliferation culture of Symplocos paniculata plantlets in vitro had been studied. [Results] The results suggested that proliferation coefficient was 5.90 in WPM with 1.7 mg/LZT. Proliferation coefficient reached 6.63 when using stems of double buds as culture materials. Culture temperature of 25/15℃ （day/night） was suitable for the proliferation of plantlets in vitro. 10 degree difference in temperature between day and night could increase proliferation coefficient up to 6.99. The seedling grown healthily. [Conclusion] The results provide a theoretical basis for Symplocos paniculata factory seedling

关 键 词：白檀 试管苗 增殖 

分 类 号：S685[农业科学—观赏园艺]