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作 者:刘秋月[1] 李珂[1] 齐永秀[1] LIU Qiu-yue LIKe QI Yong-xiu(Dept. of Pharmacy,Taishan Medical University,Taian 271016, China)
出 处:《泰山医学院学报》2017年第6期626-628,共3页Journal of Taishan Medical College
摘 要:目的建立同时测定阿托伐他汀钙、非洛地平与硫酸氢氯吡格雷制剂含量的方法。方法采用高效液相色谱法:色谱柱:Hypersil ODS2-C18(250 mm×4.6 mm,5μm),流动相:甲醇-乙酸铵醋酸缓冲溶液(pH=4.0)(75:25),流速:1.0 ml/min,检测波长:254 nm,进样量:20μl,柱温:30℃,内标物:甲睾酮。结果阿托伐他汀钙、非洛地平、硫酸氢氯吡格雷在5.0~40.0μg/ml(r分别为0.9997、0.9999、0.9999)浓度范围内与峰面积呈良好的线性关系;平均加样回收率分别为101.52%(RSD=2.85%,n=9)、98.21%(RSD=2.94%,n=9)、96.45%(RSD=2.64%,n=9);标示量百分含量分别为107.7%、98.15%、108.2%。结论该方法简便、准确、快速、重复性好,可用于同时测定阿托伐他汀钙与非洛地平、硫酸氢氯吡格雷制剂的含量。Objective: To establish a method for simultaneous determination of atorvastatin,felodipine,and clopidogrel hydrogen sulfate. Methods: HPLC method was used as follow: chromatographic column: Hypersil ODS2-C18(250 mm × 4. 6mm,5 μm),mobile phase: methanol-ammonium acetate(pH = 4. 0)(75: 25),the flow rate : 1. 0 ml/min,detection wavelength : 254 nm,column temperature: 30 ℃,the internal standard : Methyltestosterone. Results: The linear ranges for atorvastatin,felodipine and clopidogrel hydrogen sulfate was 5. 0 ~ 40. 0 μg/ml(r = 0. 9997,0. 9999,0. 9999); the average recoveries were 101. 52%(RSD = 2. 85%,n = 9) 、98. 21 %(RSD = 2. 94%,n = 9) 、96. 45%(RSD = 2. 64%,n = 9); the content of the marker was 107. 7%,98. 15%,108. 2% respectively. Conclusion: The method is simple,accurate,rapid and has good repeatability,and can be used for the simultaneous determination of atorvastatin,felodipine,and clopidogrel hydrogen sulfate.
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