鸡传染性贫血病病毒与三种致家禽肿瘤病毒混合感染的流行病学研究  

Epidemiological Study of Co-infection of Chicken Infectious Anemia Virus and Three Oncogenic Viruses

作　　者：王仙[1,2] 闫彩虹[1,2] 羊扬[1,2] 金文杰[1,2] WANG Xian YAN Caihong YANG Yang JIN Wenjie(Key Laboratory of Poultry Preventive Medicine of Jiangsu Province, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009 Jiangsu Co-innovation Center for Provention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009)

机构地区：[1]扬州大学兽医学院,教育部禽类预防医学重点实验室,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国家禽》2017年第13期21-25,共5页China Poultry

基　　金：江苏省“青蓝工程”项目;江苏高校优势学科建设工程项目(PAPD)

摘　　要：根据鸡传染性贫血病病毒(CIAV)、马立克氏病病毒(MDV)、网状内皮组织增生症病毒(REV)、禽白血病病毒(ALV)基因保守序列各设计1对特异性引物,采集102份发病家禽的肝脏、脾脏、胸腺和法氏囊等病料,通过RT-PCR和PCR方法进行检测。结果显示:CIAV、MDV、REV、ALV单一感染检出率分别为23.5%(24/102)、4.9%(5/102)、1.0%(1/102)、8.8%(9/102);CIAV和MDV、CIAV和REV、CIAV和ALV、MDV和REV、MDV和ALV、REV和ALV二重感染率分别为2.0%(2/102)、4.9%(5/102)、10.8%(11/102)、2.0%(2/102)、2.0%(2/102)、1.0%(1/102);三重感染中CIAV、MDV和REV为0(0/102),CIAV、MDV和ALV为3.9%(4/102),CIAV、REV和ALV为7.9%(8/102),MDV、REV和ALV为1.0%(1/102);四重感染率为1.0%(1/102)。26份样品中四种病毒均未检出。CIAV阳性病料与阴性病料相对应的REV共感染率具有极显著差异(P<0.01),CIAV阳性病料与阴性病料相对应的ALV共感染率具有显著差异(0.01<P<0.05)。研究结果进一步丰富了四种病毒的流行病学数据。According to the conserved viral gene sequences of chicken infectious anemia virus （CIAV）, Marek＇s disease virus （MDV）, reticuloendotheliosis virus （REV） and avian Leucosis virus （ALV）, pairs of specific primers were designed for PCR and RT-PCR detection of these 4 virus in collected field samples ti＇om chicken. The results indicated that the checkout ratios of the four viruses （CIAV/MDV/REV/ALV） were 23.5%（24/102）, 4.9%（5/102）, 1.0%（1/102） and 8.8% （9/102）; the co-infection rates of two viruses （CIAV-MDV/CIAV-REV/CIAV-ALV/MDV-REV/MDV-ALV/REV-ALV） were 2.0%（2/i02）, 4.9%（5/102）, 10.8%（I1/102）, 2.0%（2/102）, 2.0%（2/102） and 1.0%（1/102） respectively; the co-infection rates of three virus （CIAV-MDV-REV/CIAV-MDV-ALV/CIAV-REV-ALV/MDV-REV-ALV） were 0（0/102）, 3.9%（4/102）, 7.9% （8/102） and 1.0%（1/102） respectively; the co-infection rates of all four viruses （CIAV-MDV-REV-ALV） were 1.0% （1/102）, 26 samples wasn＇t detected any of the above viruses. The results showed that the infection of CIVA was highly companied with REV （P〈0.O1） and companied with ALV （0.01〈P〈0.05）, which provided an abundant epidemic data for these four viruses.

关 键 词：鸡传染性贫血病病毒 马立克氏病病毒 网状内皮组织增生症病毒 禽白血病病毒 混合感染 PCR 

分 类 号：S855.3[农业科学—临床兽医学]