脂多糖对人鼻黏膜上皮细胞人β防御素2诱导表达的影响  

Roles of lipopolysaccharide in the expression of human β defensin 2 in human nasal mucosa epithelial cells

作　　者：胡秀娟[1] 刘海兵[1] 贺广湘[2] HU Xiu-juan LIU Hai-bing HE Guang-xiang(Department of Otolaryngology Head and Neck Surgery, the Affiliated Hospital of Chengdu University, Chengdu 610081, China)

机构地区：[1]成都大学附属医院耳鼻咽喉头颈外科,成都610081 [2]中南大学湘雅三医院耳鼻咽喉头颈外科,长沙410013

出　　处：《中国眼耳鼻喉科杂志》2017年第4期245-248,共4页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：湖南省自然科学基金项目(14JJ2038);湖南省长沙市科技项目(K1203050-31)

摘　　要：目的观察脂多糖(LPS)诱导人鼻黏膜上皮细胞人β防御素2(h BD-2)表达的影响。方法用LPS刺激人鼻黏膜上皮细胞,反转录聚合酶链反应(RT-PCR)检测h BD-2 mRNA的表达,免疫细胞化学检测h BD-2蛋白的表达。结果 LPS刺激2 h后人鼻黏膜上皮细胞可见h BD-2 mRNA的表达,并呈剂量和时间依赖性;刺激4 h后人鼻黏膜上皮细胞胞质中可见h BD-2蛋白的表达。结论一定剂量的LPS可诱导人鼻黏膜上皮细胞h BD-2的表达,这种表达具有一定的剂量和时间依赖性。Objective To explore the role of lipopolysaceharide （LPS） in the expression of human β defensin 2 （hBD-2） in human nasal mucosa epithelial cells. Methods After the human nasal mucosa epithelial cells were stimulated with LPS, the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR） and the expression of hBD-2 protein was deteeted by immunoeytochemistry. Results The hBD-2 mRNA could be detected 2 hours after LPS stimulation and expressed in a dose- and time-dependent manner. The hBD-2 protein could be detected in cytoplasm 4 hours after LPS stimulation. Conclusions A certain dose of LPS could induce the expression of hBD-2 in human nasal mueosa epithelial cells in a dose- and time-dependent manner.

关 键 词：人鼻黏膜上皮细胞 人Β防御素2 脂多糖 

分 类 号：R765[医药卫生—耳鼻咽喉科]