早期皮损组织液的定量PCR检测在梅毒诊断中的价值分析  

The value of quantitative PCR detection in early skin tissue fluid of syphilis

作　　者：吴丽婷[1] 欧武英[2] 马乙云[1] 

机构地区：[1]海南省人民医院门诊部,海口571100 [2]海口市人民医院检验科,570208

出　　处：《国际检验医学杂志》2017年第14期1942-1944,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨早期皮损组织液的荧光定量聚合酶链反应(FQ-PCR)检测在梅毒诊断中的价值。方法选择2014年9月至2016年9月该院收治的40例疑似梅毒患者,患者在判断为疑似病例后,于次日清晨抽取5mL静脉血送至免疫实验室,进行甲苯胺红不加热血清试验(TRUST)、梅毒螺旋体抗体ELISA检测;并收集皮损组织液,进行FQ-PCR检测;均采用苄星青霉素治疗3、6个月后再次检测,记录转阴率。结果 ELISA、FQ-PCR总检出率比较,差异无统计学意义[97.50%(39/40)与95.00%(38/40),P>0.05];ELISA、FQ-PCR总检出率均明显比TRUST总检出率高,差异有统计学意义[97.50%(39/40)与67.50%(27/40),95.00%(38/40)与67.50%(27/40),P<0.05];在治疗后3个月、6个月时,ELISA、FQ-PCR转阴率均明显高于TRUST,差异有统计学意义(P<0.05),FQ-PCR转阴率和ELISA比较,差异无统计学意义(P>0.05);在FQ-PCR检测结果中,治疗后TP-DNA定量平均值均得到明显降低,Ⅰ期、Ⅱ期和治疗前比较,差异有统计学意义(P<0.05)。结论采用早期皮损组织液FQ-PCR可有效测定梅毒螺旋体DNA,有利于梅毒诊断,临床应用价值高。Objective To study the value of fluorescence quantitative polymerase chain reaction（FQ-PCR）detection in early skin tissue fluid of syphilis.Methods A total of 40 patients of suspected syphilis who received therapy from September 2014 to September 2016 in our hospital were selected in this study.Five mL venous blood samples were collected in all the patients,and detected by toluidine red unheated serum test（TRUST）and treponema pallidum antibody enzyme-linked immunosorbent assay（ELISA）,and skin tissue fluid were collected and performed FQ-PCR detection,all patients were treated with benzylpenicillin for 3 and 6 months,then detected again,the conversion rates were record.Results There was no significant difference in the detection rate of ELISA and FQ-PCR[97.50%（39/40）vs.95.00%（38/40）,P〉0.05].The total detection rate of ELISA and FQ-PCR were significantly higher than that of TRUST[97.50%（39/40）vs.67.50%（27/40）,95.00%（38/40）vs.67.50%（27/40）,P〈0.05].After 3 and 6 months of treatment,negative rates of ELISA and FQ-PCR were significantly higher than that of TRUST（P〈0.05）,there was no significant difference on negative rate between FQ-PCR and ELISA（P〉0.05）.In the FQ-PCR detection results,the average value of TP-DNA was significantly decreased after treatment,there were significant differences in the phase Ⅰ,phase Ⅱcompared with before treatment（P〈0.05）.Conclusion FQ-PCR could be used to measure treponema pallidum（TP-DNA）effectively in early stage,it′s conducive to the diagnosis of syphilis,the clinical application value is high.

关 键 词：梅毒 组织液 荧光定量聚合酶链反应 梅毒螺旋体 

分 类 号：R440[医药卫生—诊断学] R759.1[医药卫生—临床医学]