重离子~7Li辐射对玉米还原糖含量的影响研究  

Effects of heavy ion ~7Li irradiation on maize reducing sugar content

作　　者：胡金山[1,2] 耿金鹏[1] 曹天光[1] 安海龙[1] 展永[1] HU Jinshan GENG Jinpeng CAO Tianguang AN Hailong ZHAN Yong(Institute of Biophysics, Hebei University of Technology, Tianjin 300401, China College of Chemical Engineering, North China University of Science and Technology, Hebei Tangshan 063009, China)

机构地区：[1]河北工业大学生物物理研究所,天津300401 [2]华北理工大学化学工程学院,河北唐山063009

出　　处：《河北工业大学学报》2017年第3期8-12,共5页Journal of Hebei University of Technology

基　　金：河北省自然科学基金(B2014209314;C2013202192);河北省高等学校科学技术研究项目(ZD2017023);唐山市科技计划(13130225z)

摘　　要：研究重离子~7Li辐射对"永19"玉米自交系还原糖含量的影响.采用溴化钾压片法红外光谱定性表征玉米还原糖.优化3,5-二硝基水杨酸(DNS)法的最佳波长,在540 nm定量测定不同剂量重离子7Li辐射自交系的还原糖含量.提取液与标准品特征峰重合证明提取液为还原糖.定量结果表明10 Gy辐射还原糖含量高达4.50%,20和40 Gy辐射分别为3.94%、3.79%,而30和50 Gy辐射略低于对照(3.55%).重离子~7Li辐射对玉米还原糖含量有正负双重作用呈现出马鞍形曲线.An investigation demonstrated the effects of heavy ion 7Li irradiation on ＂Yong 19＂ maize inbred lines＂ reduc- ing sugar content. Using KBr tableting technique, maize redueing sugar was qualitatively characterized by infrared spec- trum. Then, optimized the wavelength for 3, 5-dinitro salicylic acid （DNS） determination, all the reducing sugars from dif- ferent doses of heavy ion 7Li treated inbred lines were quantitatively measured at 540 nm. These eharacteristic peaks of ex- traction and standard were eoineident, whieh proved that the extraction was reducing sugar. The quantitative results showed that reducing sugar content of 10 Gy radiation reached as high as 4.50%, 20 and 40 Gy radiation were 3.94% and 3.79%, whereas 30 and 50 Gy radiation were slightly lower than that of the eontrol sample （3.55%）. It indicated that heavy ion 7Li irradiation has both positive and negative effects on maize reducing sugar content, which presented a kind of sad- dle-type curve.

关 键 词：重离子7Li 辐射 还原糖 红外表征 3 5-二硝基水杨酸 

分 类 号：Q691[生物学—生物物理学]