基于均匀设计法评价白及制川乌配伍对胃溃疡模型小鼠的影响  

The anti-gastric ulcer effects of combined administration of Bletilla striata and Radix aconiti preparata in mice on the basis of uniform design

作　　者：孙丛丛[1] 刘春芳[2] 孙丹妮[2] 汪倩倩[2] 王慧[2] 何莲花[2] 林娜[2] Sun Congcong Liu Chunfang Sun Danni Wang Qianqian Wang Hui He Lianhua Lin Na(Chengde Medical University, Chengde 067000, Chin)

机构地区：[1]承德医学院研究生学院,067000 [2]中国中医科学院中药研究所,北京100700

出　　处：《国际中医中药杂志》2017年第7期611-616,共6页International Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划（2011CB505300、2011CB505305）;国家自然科学基金（81173

摘　　要：目的 研究白及与制川乌不同配比和剂量变化对无水乙醇致胃溃疡模型小鼠胃黏膜损伤的影响.方法 采用均匀设计法,按2因素7水平,以小鼠胃溃疡指数和胃黏膜病理损伤积分为指标,观察白及制川乌合煎液灌胃给药后对胃溃疡小鼠的影响,并选取有显著意义的配比和剂量进行验证.结果 白及制川乌总剂量在3.64~29.32 g/kg范围内,小鼠溃疡指数和病理损伤积分随合煎液中白及剂量的增加而降低、随制川乌剂量的增加而升高,但二药未见交互作用;当总剂量固定不变,小鼠溃疡指数和病理损伤积分即随合煎液白及配比增加而降低、随制川乌配比增加而升高.结论 白及和制川乌合煎液对小鼠胃溃疡的影响与二药的剂量和配比密切相关,为十八反中白及与制川乌反药组合作用的深入研究提供实验依据.Objective To study the influence of the combined administration of Bletilla striata and Radix aconiti preparata with different doses or ratios on anhydrous ethanol-induced gastric mucosal injury in mice with gastric ulcer. Methods The uniform design method was adopted on the basis of two factors and seven levels, which aims to investigate the influence of combined oral administration of Bletilla striata and Radix aconiti preparata with different doses or ratios on gastric mucosal injury in the mice with gastric ulcer. The indices like gastric ulcer index and stomach mucosa damage index were used to help selecting the right dose or ratio of decoctions for further study. Results The combined administration of Bletilla striata and Radix aconiti preparata at a certain proportion could reduce gastric ulcer index and stomach mucosa damage index. According to the regression analysis, the gastric ulcer index and stomach mucosa damage index were obviously inhibited by Bletilla striata, while this function decreased with the combined administration of Bletilla striata and Radix aconiti preparata. However, an interactive effect between each other has not been found. The influence of decoctions on the gastric ulcer index and stomach mucosa damage index became less when the compatibility ratio of Radix aconiti preparata increased, which referred to the total dose ranged between 3.64 g/kg and 29.32 g/kg. Conclusions The effect of the combined administration of Bletilla striata and Radix aconiti preparata showed relationship among the doses and ratios on anhydrous ethanol-induced gastric mucosal injury in mice with gastric ulcer.

关 键 词：十八反 制川乌 白及 胃溃疡 小鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]