人源类金属硫蛋白能促进番茄幼苗对锌的积累  

Homo sapiens Metallothionein 1L (HsMT1L) Enhances the Accumulation of Zinc in Tomato Seedlings

作　　者：宋小梅[1] 陈梦莹[1] 秦小冬[1] 金新开 李金华[1] 潘宇[1] 苏承刚[1] 张兴国[1] SONG Xiao-mei CHEN Meng-ying QIN Xiao-dong LI Jin-hua PAN Yu SU Cheng-gang ZHANG JIN Xin-kai Xing-guo(School of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China)

机构地区：[1]西南大学园艺园林学院,重庆400716

出　　处：《西南大学学报（自然科学版）》2017年第7期30-35,共6页Journal of Southwest University(Natural Science Edition)

基　　金：科技部"863"项目(2010AA10060705);国家级大学生创新创业训练计划项目(201310635019)

摘　　要：金属硫蛋白具有抗辐射、抗氧化和对重金属解毒等功能,对Zn^(2+)具有很强的结合能力,在医药、农业、环境和保健等诸多领域有潜在的应用价值.将花椰菜花叶病毒35S启动子控制的人源类金属硫蛋白基因HsMT1L导入番茄基因组,结果显示,转基因番茄幼苗比野生型材料对Zn^(2+)具有更强的耐受性,并能够富集更多的Zn^(2+)离子.Metallothionein（MT）has the functions of radio-resistance,oxidation-resistance and heavy-metal detoxification,and has a high binding capacity to Zn^2＋,and hence it has potential application value in medicine,agriculture,environment protection,health care,and others.In this study,HsMT1L,a metallothionein 1-like gene from human,which is controlled by cauliflower mosaic virus 35 Spromoter containing duplicated enhancer sequence（2x35S）,was introduced into the genome of tomato（Solanum lycopersicum L.cv Ailsa Craig）.The results showed that the tomato seedlings containing the 2x35S-HsMT1 Lexhibited higher Zn^2＋ tolerance and higher Zn^2＋ accumulation in the plant than the wild type.

关 键 词：人源类金属硫蛋白基因 番茄 幼苗 锌 

分 类 号：Q786[生物学—分子生物学]