氯化钴化学模拟低氧与低氧环境对大鼠肺动脉成纤维细胞的作用比较  

Effects comparison of chemical hypoxia induced by cobalt chloride and hypoxia environment on rat pulmonary arterial fibroblasts

作　　者：柴晓宇[1] 许慧莹[1] 刘钟桧 易亮[1] 刘新民[1] 

机构地区：[1]北京大学第一医院老年科,北京100034

出　　处：《重庆医学》2017年第21期2889-2891,2894,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81270114)

摘　　要：目的比较氯化钴(CoCl_2)化学模拟低氧及低氧环境对于原代大鼠肺动脉成纤维细胞(PAFs)增殖、迁移、表型转化的作用。方法分离培养原代大鼠PAFs,利用CoCl_2刺激细胞,或通过低氧细胞培养(1%O_2)刺激诱导PAFs,通过CCK-8试验、细胞划痕、细胞迁移、表型转化标志蛋白及磷脂酰肌醇3激酶(PI3K)/丝氨酸苏氨酸蛋白激酶(Akt)信号通路蛋白表达的实验结果比较CoCl_2与低氧环境对于PAFs的作用。结果与对照组相比,100μmol/mL CoCl_2刺激对PAFs的细胞增殖活力、迁移能力、细胞表型转化能力的作用无明显影响(P>0.05);而1%O_2可以明显提高PAFs的细胞增殖活力和迁移活力,并可以使α-平滑肌肌动蛋白(α-SMA)表达上调(P<0.05)。结论 CoCl_2化学模拟低氧与低氧环境对PAFs的促增殖、促迁移、促表型转化作用存在差异。Objective To compare the effects of cobalt chloride （CoCl2） induced chemical hypoxia and hypoxia environment on the proliferation,migration and phenotype transformation of rat primary pulmonary artery fibroblasts （PAFs）. Methods Prima- ry PAFs were isolated and cultured. Ceils were stimulated by CoCl2 ,or hypoxia cell culture （1% O2 ） was used to stimulate and in- duce PAFs. Then the effects of COCl2 and hypoxia environment on PAFs were compared by CCK-8 assay, scratch assay,transwell assay,phenotype marker protein expression and PI3K/Akt signaling pathway protein expression. Results Compared with the con- trol group, 100 μmol/mL CoCl2 stimulation had no significant effect on the cell proliferation activity,cell migration ability and phe- notype transformation ability of PAFs （P〉0.05） ;while 1% O2 could significantly improve the cell proliferation and migration ac- tivities of PAFs as well as the upregulation of α-SMA expression （P〈0. 05）. Conclusion There exist differences of effects between CoCl2 induced chemical hypoxia and hypoxia errvironment on promoting cell proliferation, cell migration and phenotype transforma- tion in PAFs.

关 键 词：氯化钴 低氧 肺动脉 成纤维细胞 表型转化 

分 类 号：R543.2[医药卫生—心血管疾病]