新型脱细胞真皮基质的制备及相关性能实验研究  

Research on preparation and relevant performance test for new-type acellular dermal matrix

作　　者：熊武[1] 张彪[2] 蔡昀[1] 黄新灵[1] 黄巧丽 全文娟[2] 陈莹莹[1] 兰宏伟[1] 周忠志[1] 

机构地区：[1]湖南中医药大学第一附属医院烧伤疮疡整形外科,长沙410007 [2]湖南中医药大学中医学院,长沙410208

出　　处：《中国医师杂志》2017年第7期1018-1021,共4页Journal of Chinese Physician

摘　　要：目的制备一种新型脱细胞真皮基质（ADM）并研究其相关性能，为临床应用提供理论依据。方法选用巴马香猪皮为皮源，采用物理、化学及生物技术制备新型ADM。将制得的新型ADM采用HE染色观察、扫描电镜观察、氨基酸分析、材料孔隙率测试、亲水性测试、拉伸强度测试、体外降解试验、细胞毒性试验及动物实验检测其外观结构、理化性能和生物性能。结果新型ADM细胞去除彻底，真皮结构完整，胶原蛋白（CP）含量达95．55％，具有连通的三维孔状结构，其孔隙率达（85．03±0．99）％，接触角为（24．56±0．57）°，提示新型ADM为亲水物质，拉伸强度为（5．48±0．44）Pa，提示其拉力适中，体外降解时间缩短为（28．7±0．76）h，细胞相对增殖率（RGR）〉75％，细胞在新型ADM上生长增殖良好，降解后能被原有组织替代。结论新型ADM克服了传统制备方法破坏真皮基质结构和基质中细胞脱洗不彻底的缺点，具有较高的CP和孔隙率，其生物性能得到改善，炎症免疫反应降低，降解速率加快，临床应用价值较高。Objective To prepare a new-type acellular dermal matrix （ADM） and research on its relevant performance, which would provide theoretical evidence for clinical application. Methods Skin of Bama suckling pig was taken as resource of skin, and technologies of physics, chemistry and biology were selected to prepare new-type ADM. To detect the external structure, physical and chemical property as well as biological property of the prepared new-type ADM, hematoxylin-eosin （HE） staining observation, scan- ning electron microscope observation, amino acid analysis, material porosity and hydrophilicity test, tensile strength and in vitro degradation experiment, cytotoxicity test, and animal experiment have been conducted. Results New-type ADM cells have been thoroughly removed and dermal matrix remains intact with collagen content of 95.55% , connective three-dimensional pore structure, （85.03±0. 99）% of porosity, （24. 56±0. 57）° of contact angle implying new-type ADM was hydrophilie substance, （ 5.48±0. 44 ） Pa of tensile strength implying its moderate level of pulling force, in vitro degradation period reduced to （28. 7±0. 76） h, and 〉75% relative growth rate （RGR）. Cells grew and proliferated on new-type ADM and could be replaced by original tissue after degradation. Conclusions New-type ADM have overcome disadvantages of traditional preparation method in sabotaging dermal matrix structure and incompletely removing cells from matrix, which is qualified with higher level of collagen content and porosity. With improved biological property, greatly reduced inflammation immunoreactions, and accelerated degradation rate, new-type ADM is of higher level of clinical application value.

关 键 词：真皮/细胞学 细胞外基质 

分 类 号：R318.08[医药卫生—生物医学工程]