microRNA-143对小鼠失神经肌肉萎缩中肌纤维的影响  

Effect of MicroRNA-143 on Myofiber of Denevated Muscle Atrophy in Mouse

作　　者：张会娜[1] 张蔚然[1] 王轶敏[1] 刘新峰[1] 郭宏[1] 丁向彬[1] 

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农学院学报》2017年第2期39-43,共5页Journal of Tianjin Agricultural University

基　　金：国家自然科学基金资助项目"牛骨骼肌卫星细胞成肌分化相关miRNAs的鉴定和功能研究"(31201021);国家大学生创新创业训练计划项目(201510061006);天津市"131"创新型人才培养工程第二层次人选资助项目(无编号)

摘　　要：为研究microRNA-143(miR-143)对小鼠失神经肌肉萎缩中肌纤维的影响,通过对小鼠进行坐骨神经切断术,构建小鼠腓肠肌失神经萎缩模型,并注射miR-143模拟物或miR-143抑制剂,采用实时定量PCR检测miR-143的表达量,比较肌肉湿重和肌纤维直径,研究miR-143对小鼠失神经腓肠肌的调控作用。结果显示:注射miR-143模拟物的小鼠,其肌纤维直径明显低于对照组,而注射miR-143抑制剂的小鼠,其失神经腓肠肌组织肌纤维的直径明显高于对照组。结果说明,miR-143对小鼠失神经腓肠肌肌肉萎缩的修复起着一定调节作用。To investigate the effects of microRNA-143 （miR-143） on denervated muscle atrophy in mouse, an amyotrophia mouse model of sciatic nerve resection was established, l0 nmol miR-143 mimics or 10 nmol miR-143 inhibitors were injected to denevated gastrocnemius. Then, the expression of miR-143 was detected by qRT-PCR, the wet weight of gastrocnemius and the cross section diameter of the muscle fibers were compared between the atrophying skeletal muscle and control. The results showed that the diameter of myofiber injected with miR-i 43 mimics was significantly lower than that of control group while the diameter of myofiber injected with miR-143 inhibitors was significantly higher than that of control group. The results suggested that miR-143 plaved a role in reoairing denervated muscle atrophy.

关 键 词：microRNA-143 小鼠 失神经 肌肉萎缩 肌纤维 腓肠肌 

分 类 号：P641.131[天文地球—地质矿产勘探] S152.72[天文地球—地质学]