微拟球藻优化培养模式及其在光生物反应器高密度培养中的应用  

OPTIMIZED CULTURE MODE AND ITS APPLICATION FOR DENSE CULTURE OF NANNOCHLOROPSIS SALINA IN DIFFERENT PHOTOBIOREACTORS

作　　者：刘建国[1] 张京浦[1] 殷明焱[1] 孟昭才[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学集刊》2007年第1期55-66,共12页Studia Marina Sinica

基　　金：中国科学院方向性创新重要方向项目KZCXZ-YW-209(L48032409D);山东省科技项目(98-18)资助

摘　　要：The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.The impacts of different factors (including light, temperature, nitrogen, phosphorous, iron, carbon, zinc, boron, cobalt, copper, molybdenum, manganess, etc) on cell growth of Nannochloropsis salina, the sole marine genus of the Eustigmatophyceae, were carried out in Qingdao and Sigapore with nature seawater. Based on the results obtained, a optimum model good for culturing this unicellular alga was suggested as follows: 120mg/L urea, 6mg/L KH2PO4, 1.5 mg/L Ferric citrate, 0.05 mg/L ZnSO4, 0.01 mg/L CuSO4, 0.1 mg/L NaMoO3, 0.02 mg/L CoCl2, 0.5 mg/L HBO3, 1.0 mg/L MnSO4,25‰～30‰NaCl, continuous illumination indoor or suitable strong light intensity as well as a contant temperature between 25～30℃, or varied temperature between 25～35℃, specific growth rate at 10% ～15%.3.6×1012 to 1.2×1011 cells /L of dense culture of Nannochloropsis salina were achieved by using 10L of tubular coiled photobioreactor,60L, 200L, 500L and 1600 L of flat glass photoreactors, respectively, which was several hundred-fold higher than the cell density of traditional microalgal culture in aquaculture. Specific growth rate, negative correlation with cell density, affected the biomass output in Nannochloropsis salina culture system. 10%～15% of specific growth rate was suggested in application for maximal production.

关 键 词：光生物反应器 高密度培养 比生长速率 生物产量 光径 天然海水 海洋微藻 细胞密度 水产养殖 适宜范 

分 类 号：Q943.1[生物学—植物学]