HPLC法同时测定黄芪药材中10种黄酮类成分的含量  

Simultaneous Determination of 10 Flavonoids in Astragalus membranaceus by HPLC

作　　者：张妍[1,2] 董琳[1] 雍婧姣 毛福英[1] 尹蕾[1] 付雪艳[1] ZHANG Yan DONG Lin YONG Jingjiao MAO Fuying YIN Lei FU Xueyan(Ningxia Research Center for Modem Engineering and Technology of Hui Medicine, Ningxia Medical University, Yinchuan 750001, China Ningxia Key Lab of Hui Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750001, China)

机构地区：[1]宁夏医科大学宁夏回药现代化工程技术研究中心,银川750001 [2]宁夏医科大学宁夏回医药现代化省部共建教育部重点实验室,银川750001

出　　处：《中国药房》2017年第21期2970-2973,共4页China Pharmacy

基　　金：宁夏自然科学基金资助项目(No.NZ14060)

摘　　要：目的:建立同时测定黄芪药材中10种黄酮类成分含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent SB-C_(18),流动相为乙腈-0.3%甲酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为254 nm,柱温为35℃,进样量为10μL。结果:毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、染料木苷、刺芒柄花苷、毛蕊异黄酮、槲皮素、染料木素、山柰酚、异鼠李素和芒炳花素检测进样量线性范围分别为0.030 29~1.514 5μg(r=0.999 4)、0.015 00~0.7 500μg(r=0.999 5)、0.007 39~0.369 5μg(r=0.999 1)、0.12011~6.005 5μg(r=0.999 8)、0.038 36~1.918μg(r=0.999 9)、0.029 89~1.494 5μg(r=0.999 5)、0.007 04~0.352μg(r=0.9994)、0.016 83~0.841 5μg(r=0.999 5)、0.004 54~0.227μg(r=0.999 9)、0.013 36~0.668μg(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为99.55%~100.45%(RSD=0.36%,n=6)、99.34%~101.00%(RSD=0.59%,n=6)、98.05%~100.36%(RSD=1.27%,n=6)、99.73%~100.13%(RSD=0.18%,n=6)、99.70%~100.30%(RSD=0.22%,n=6)、99.67%~103.27%(RSD=1.37%,n=6)、98.13%~104.41%(RSD=2.37%,n=6)、96.35%~100.06%(RSD=1.46%,n=6)、99.47%~101.13%(RSD=0.60%,n=6)、99.70%~100.06%(RSD=0.15%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于黄芪药材中10种黄酮类成分含量的同时测定。OBJECTIVE： To establish a method for simultaneous determination of 10 flavonoids in Astragalus membranaceus. METHODS： HPLC method was adopted. The determination was performed on Agilent SB-C18 column with mobile phase consisted of acetonitrile-0.3% formic acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS： The linear ranges of calycosin-7-O-glucoside, isoquercitrin, genistin, ononin, calycosin, quercetin, genistein, kaempferol, isorhamnetin and formononetion were 0.030 29-1.514 5 μg（r=0.999 4）,0.015 00-0.7 500 μg（r=0.999 5）,0.007 39-0.369 5 μg（r=0.999 1）,0.120 11-6.005 5 μg（r=0.999 8）,0.038 36-1.918 μg（r=0.999 9）,0.029 89-1.494 5 μg（r=0.999 5）,0.007 04-0.352 μg（r=0.999 4）,0.016 83-0.841 5 μg（r=0.999 5）,0.004 54-0.227 μg（r=0.999 9）, 0.013 36-0.668 μg（r=0.999 9）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 99.55%-100.45%（RSD=0.36%,n=6）,99.34%-101.00%（RSD=0.59%,n=6）, 98.05%-100.36%（RSD=1.27%,n=6）, 99.73%-100.13%（RSD=0.18%,n=6）,99.70%-100.30%（RSD=0.22%,n=6）,99.67%-103.27%（RSD=1.37%,n=6）, 98.13%-104.41%（RSD=2.37%,n=6）,96.35%-100.06%（RSD=1.46%,n=6）, 99.47%-101.13%（RSD=0.60%,n=6）, 99.70%-100.06%（RSD=0.15%,n=6）, respectively. CONCLUSIONS： This method is convenient, sensitive, stable and reproducible, can be used for simultaneous determination of 10 flavonoids in A. membranaceus.

关 键 词：黄芪 黄酮类成分 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]