RP-HPLC法同时测定抗菌消炎片中7种成分的含量  

Simultaneous Determination of 7 Components in Kangjun Xiaoyan Tablets by RP-HPLC

作　　者：许丽丽 王玉团 徐丽华 林永强 牛艳 XU Lili WANG Yutuan XU Lihua LIN Yongqiang NIU Yan(Shandong Institute for Food and Drug Control, Jinan 250101, China)

机构地区：[1]山东省食品药品检验研究院,济南250101

出　　处：《中国药房》2017年第21期2981-2984,共4页China Pharmacy

基　　金：山东省自然科学基金资助项目(No.ZR2013HM074)

摘　　要：目的:建立同时测定抗菌消炎片中绿原酸、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:采用反向高效液相色谱法。色谱柱为Agilent Extend C_(18),流动相为乙腈-0.5%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长分别为327 nm(绿原酸)、280 nm(黄芩苷)和450 nm(芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚),柱温为30℃,进样量为10μL。结果:绿原酸、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚检测进样量线性范围分别为0.209 4~4.188 0μg(r=0.999 9)、0.372 0~7.440 0μg(r=0.999 9)、0.006 3~0.126 2μg(r=0.999 9)、0.011 6~0.232 2μg(r=0.999 9)、0.005 3~0.106 0μg(r=0.999 8)、0.012 8~0.256 4μg(r=0.999 9)、0.016 5~0.330 3μg(r=0.999 8);定量限分别为2.01、1.93、0.76、1.25、1.24、0.53、1.53 ng,检测限分别为0.98、0.65、0.25、0.42、0.41、0.18、0.52 ng;加样回收率分别为98.41%~100.40%(RSD=0.76%,n=9)、96.17%~100.10%(RSD=1.58%,n=9)、96.11%~100.10%(RSD=1.33%,n=9)、96.29%~100.80%(RSD=1.85%,n=9)、96.88%~100.10%(RSD=1.22%,n=9)、97.81%~101.90%(RSD=1.64%,n=9)、96.46%~101.30%(RSD=1.85%,n=9)。结论:该方法准确可靠,重复性好,可用于抗菌消炎片中7种成分含量的同时测定。OBJECTIVE： To develop a method for simultaneous determination of chlorogenic acid, baicalin, aloe emodin, rhein, emodin, chrysophanol and physcione in Kangjun xiaoyan tablets. METHODS： RP-HPLC method was adopted. The determination was performed on Agilent Extend C18 with mobile phase consisted of acetonitrile-0.5% phosohoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 327 nm （chlorogenic acid）, 280 nm （baicalin） and 450 nm （aloe emodin, rhein, emodin, chrysophanol, physcione）. The column temperature was 30 ℃, and sample size was 10 μL. RESULTS： The linear ranges of chlorogenic acid, baicalin, aloe emodin, rhein, emodin, chrysophanol and physcione were 0.209 4-4.188 0 μg（r=0.999 9）,0.372 0-7.440 0 μg（r=0.999 9）,0.006 3-0.126 2 μg（r=0.999 9）,0.011 6-0.232 2 μg（r=0.999 9）,0.005 3- 0.106 0 μg（r=0.999 8）, 0.012 8-0.256 4 μg（r=0.999 9）, 0.016 5-0.330 3 μg（r=0.999 8）, respectively. The limits of quantitation were 2.01, 1.93, 0.76, 1.25, 1.24, 0.53 and 1.53 ng; the limits of detection were 0.98, 0.65, 0.25, 0.42, 0.41, 0.18 and 0.52 ng, respectively. The recoveries were 98.41%-100.40%（RSD=0.76%,n=9）,96.17%-100.10%（RSD=1.58%,n=9）,96.11%-100.10%（RSD=1.33%,n=9）, 96.29%-100.80%（RSD=1.85%,n=9）,96.88%-100.10%（RSD=1.22%,n=9）,97.81%-101.90%（RSD=1.64%,n=9）, 96.46%-101.30%（RSD=1.85%,n=9）, respectively. CONCLUSIONS： The method is accurate, reliable and reproducible. It can be used for simultaneous determination of 7 components in Kangjun xiaoyan tablets.

关 键 词：抗菌消炎片 反向高效液相色谱法 绿原酸 黄芩苷 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 含量测定 

分 类 号：R927.2[医药卫生—药学]