毛重楼药材的质量标准研究  

Study on Quality Standard of Paris pubescens

作　　者：袁会琼 刘江 夏从龙 YUAN Huiqiong LIU Jiang XIA Conglong(College of Pharmacy and Chemistry, Dali University, Yunnan Dali 671000, China)

机构地区：[1]大理大学药学与化学学院,云南大理671000

出　　处：《中国药房》2017年第21期2985-2988,共4页China Pharmacy

基　　金：云南省应用基础研究计划项目(No.2013FB059)

摘　　要：目的:建立毛重楼药材的质量标准。方法:从原植物形态、性状特征、显微特征(横切面、粉末)对毛重楼药材进行定性鉴别;采用高效液相色谱法测定药材中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量:色谱柱为Agilent C_(18),流动相为乙腈-水(梯度洗脱),流速为0.9mL/min,检测波长为203 nm,柱温为25℃,进样量为5μL;采用紫外分光光度法测定药材中重楼总皂苷的含量。结果:毛重楼根状茎呈结节状扁圆柱形,略弯曲。横切面木质部导管较大,韧皮部细胞小,粉末有大量淀粉粒,多为单粒。重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ和重楼总皂苷检测进样量线性范围分别为0.64~12.8、0.46~9.2、0.26~5.2、0.23~4.6、20.5~143.5μg(r分别为0.999 9、0.999 9、0.999 9、0.999 9、0.999 7);精密度、稳定性、重复性试验的RSD<3.0%;加样回收率分别为96.38%~105.24%(RSD=3.01%,n=6)、97.24%~102.57%(RSD=2.50%,n=6)、97.19%~101.74%(RSD=1.52%,n=6)、93.72%~104.00%(RSD=3.53%,n=6)、98.11%~104.50%(RSD=2.57%,n=6)。结论:该研究所建标准可用于毛重楼药材的质量控制。OBJECTIVE： To establish the quality standard of Paris pubescens. METHODS： Qualitative identification of medicinal material was conducted from original plant morphology, properties and microscopic characteristics （cross section, powder）. The contents of parissaponinⅠ,Ⅱ,Ⅵ,Ⅶ were determined by HPLC. The determination was performed on Agilent C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 0.9 mL/min. The detection wavelength was set at 203 nm, and column temperature was 25 ℃. The sample size was 5 μL. The content of total saponins in P. pubescens was determined by UV spectrophotometry. RESULTS： The rhizome of P. pubescens was nodular flat cylindrical in shape, slightly curved, tiny odor, bitter in taste. The large vessels were found in transverse xylem; phloem cells were small; powder had a large number of starch grains, mostly single grain. The linear ranges of parissaponin Ⅰ,Ⅱ,Ⅵ,Ⅶ and total saponins were 0.64-12.8, 0.46-9.2, 0.26-5.2, 0.23-4.6, 20.5-143.5 μg（r were 0.999 9、0.999 9、0.999 9、0.999 9、0.999 7）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 3.0%.The recoveries were 96.38%-105.24%（RSD=3.01%,n=6）,97.24%-102.57%（RSD=2.50%,n=6）,97.19%-101.74%（RSD=1.52%,n=6）,93.72%-104.00%（RSD=3.53%,n=6）,98.11%-104.50%（RSD=2.57%,n=6）. CONCLUSIONS： Established standard can be used for quality evaluation of P. pubescens.

关 键 词：毛重楼 药材鉴定 含量分析 重楼皂苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]