慢性阻塞性肺疾病患者外周血单个核细胞NOD2 mRNA表达及白细胞介素-6的水平及意义  

Expression of NOD2 mRNA in peripheral blood monocytes and serum interleukin-6 level in patients with chronic obstructive pulmonary disease

作　　者：贾钦尧 陈绍平[1] 李新胜[1] 宋珊[1] 毛建[1] 韩美玲[1] 

机构地区：[1]川北医学院附属医院呼吸内科,四川南充637000

出　　处：《中国老年学杂志》2017年第14期3529-3531,共3页Chinese Journal of Gerontology

基　　金：四川省教育厅科研基金项目(No.13ZA0216)

摘　　要：目的探讨外周血单个核细胞NOD2 mRNA和血清白细胞介素(IL)-6在慢性阻塞性肺疾病(COPD)发病机制中的作用及其与COPD患者严重程度的关系。方法对经过门诊及住院达到稳定期的COPD患者进行综合评估,分为A、B、C、D四组,每组30例,同期门诊健康体检者30例作为健康对照组,采用PCR和ELISA法分别检测COPD各组及健康对照组外周血单个核细胞核内NOD2 mRNA的表达及血清中IL-6的含量。结果 COPD稳定期患者外周血单个核细胞核内NOD2 mRNA及血清IL-6的表达均明显高于健康对照组(P<0.05)。COPD患者A、B、C、D各组中NOD2 mRNA和IL-6的表达明显高于健康对照组(P<0.05)。COPD患者D组NOD2 mRNA和IL-6的表达明显高于C组(P<0.05),C组和D组的NOD2 mRNA和IL-6的表达均明显高于A组和B组(P<0.05),A组和B组之间无统计学差异(P>0.05)。COPD患者NOD2 mRNA表达和IL-6的含量呈正相关(r=0.81,P<0.05)。结论 NOD2和IL-6都参与了COPD气道炎症的发生发展,其水平与COPD患者严重程度呈正相关。Objective To explore the expression of NOD2 mRNA in peripheral blood monocytes （PBMCs） and serum IL-6 level in the pathogenesis of chronic obstructive pulmonary disease （COPD） and the relationship with the severity of COPD.Methods Comprehensive evaluation of COPD patients in stable phase after outpatient and inpatient treatment,the patients were divided into A, B, C, D groups, 30 cases in each group, 30 healthy examiners in the outpatient on the corresponding period were chosen as healthy control group.PCR method and enzyme linked immunosorbent assay （ELISA） were respectively adopted to detect the expression of NOD2 mRNA in PBMCs and serum IL-6.Results The expression of NOD2 mRNA and serum IL-6 from the COPD patients were higher than those of healthy control group（P〈0.05）.The expression of NOD2 mRNA and serum IL-6 of D group were higher than those of C group（P〈0.05）, the expression of NOD2 mRNA and serum IL-6 of C and D group were both higher than those of A and B group（P〈0.05）.There were no statistical significant differences in the expression of NOD2 mRNA and serum IL-6 between A and B group（P〉0.05）.The expression of NOD2 mRNA and serum IL-6 in COPD patients was positively correlated （r=0.81,P〈0.05）.Conclusions NOD2 mRNA in PBMCs and serum IL-6 participate the progression of airway inflammatory response of COPD and the levels show positive correlation with disease severity.

关 键 词：慢性阻塞性肺疾病 NOD2 白细胞介素 

分 类 号：R563.3[医药卫生—呼吸系统]